Key Laboratory of Animal Resistance of Shandong Province, College of Life Sciences, Shandong Normal University, Jinan 250014, Shandong, People's Republic of China.
J Physiol Sci. 2011 Jan;61(1):37-45. doi: 10.1007/s12576-010-0119-6. Epub 2010 Dec 16.
The activity of catecholaminergic neurons in the hypothalamus and the medullary visceral zone (MVZ) in rats in response to restraint water-immersion stress (RWIS) was measured by use of dual Fos and tyrosine hydroxylase (TH) immunohistochemistry. In RWIS rats Fos immunoreactive (Fos-IR) nuclei dramatically increased in the paraventricular nucleus (PVN), the supraoptic nucleus (SON), the dorsal motor nucleus of the vagus (DMV), the nucleus of the solitary tract (NTS), the area postrema (AP), and the ventrolateral medulla (VLM). A small number of TH-immunoreactive (TH-IR) and Fos/TH double-labeling neurons in the PVN, and their absence from the SON, were observed in both RWIS and nonstressed rats. More TH-IR neurons were observed in the MVZ of RWIS rats than in nonstressed rats. In RWIS and nonstressed rats, the percentage of Fos-IR nuclei in TH-IR neurons was 38.0 and 14.3% in the DMV, 34.4 and 9.7% in the NTS, 18.6 and 4.5% in the AP, and 45.7 and 18.9% in the VLM, respectively. In conclusion, catecholaminergic neurons in the MVZ are involved in the response to RWIS; although the PVN and SON also participate in the response to RWIS, the mechanism is not via catecholaminergic neurons.
采用双重 Fos 和酪氨酸羟化酶（TH）免疫组织化学方法测量了大鼠下丘脑和延髓内脏区（MVZ）的儿茶酚胺能神经元对束缚水浸应激（RWIS）的反应活性。在 RWIS 大鼠中，室旁核（PVN）、视上核（SON）、迷走神经背核（DMV）、孤束核（NTS）、最后区（AP）和腹外侧延髓（VLM）中 Fos 免疫反应性（Fos-IR）核急剧增加。在 RWIS 和非应激大鼠中，PVN 中观察到少量的 TH-免疫反应性（TH-IR）和 Fos/TH 双标记神经元，而 SON 中则没有。RWIS 大鼠 MVZ 中的 TH-IR 神经元数量多于非应激大鼠。在 RWIS 和非应激大鼠中，DMV 中 Fos-IR 核在 TH-IR 神经元中的百分比分别为 38.0%和 14.3%，NTS 中分别为 34.4%和 9.7%，AP 中分别为 18.6%和 4.5%，VLM 中分别为 45.7%和 18.9%。总之，MVZ 中的儿茶酚胺能神经元参与了对 RWIS 的反应；尽管 PVN 和 SON 也参与了对 RWIS 的反应，但机制不是通过儿茶酚胺能神经元。
