Key Laboratory of Animal Resistance of Shandong Province, College of Life Sciences, Shandong Normal University, Shandong Province, People's Republic of China.
PLoS One. 2011;6(8):e23362. doi: 10.1371/journal.pone.0023362. Epub 2011 Aug 17.
Vasopressin (AVP) and oxytocin (OT) are considered to be related to gastric functions and the regulation of stress response. The present study was to study the role of vasopressinergic and oxytocinergic neurons during the restraint water-immersion stress.
Ten male Wistar rats were divided into two groups, control and RWIS for 1h. The brain sections were treated with a dual immunohistochemistry of Fos and oxytocin (OT) or vasopressin (AVP) or OT receptor or AVP 1b receptor (V(1b)R).
(1) Fos-immunoreactive (Fos-IR) neurons dramatically increased in the hypothalamic paraventricular nucleus (PVN), the supraoptic nucleus (SON), the nucleus of solitary tract (NTS) and motor nucleus of the vagus (DMV) in the RWIS rats; (2) OT-immunoreactive (OT-IR) neurons were mainly observed in the medial magnocellular part of the PVN and the dorsal portion of the SON, while AVP-immunoreactive (AVP-IR) neurons mainly distributed in the magnocellular part of the PVN and the ventral portion of the SON. In the RWIS rats, Fos-IR neurons were identified in 31% of OT-IR neurons and 40% of AVP-IR neurons in the PVN, while in the SON it represented 28%, 53% respectively; (3) V(1b)R-IR and OTR-IR neurons occupied all portions of the NTS and DMV. In the RWIS rats, more than 10% of OTR-IR and V(1b)R-IR neurons were activated in the DMV, while lower ratio in the NTS.
RWIS activates both oxytocinergic and vasopressinergic neurons in the PVN and SON, which may project to the NTS or DMV mediating the activity of the neurons by OTR and V(1b)R.
加压素 (AVP) 和催产素 (OT) 被认为与胃功能和应激反应的调节有关。本研究旨在研究束缚水浸应激过程中加压素能和催产素能神经元的作用。
将 10 只雄性 Wistar 大鼠分为对照组和 RWIS 组，每组 1h。用 Fos 和催产素 (OT) 或加压素 (AVP) 或 OT 受体或 AVP1b 受体 (V(1b)R) 的双重免疫组织化学方法处理脑切片。
(1) RWIS 大鼠下丘脑室旁核 (PVN)、视上核 (SON)、孤束核 (NTS)和迷走神经运动核 (DMV) 中 Fos 免疫反应性 (Fos-IR) 神经元显著增加；(2)OT-IR 神经元主要存在于 PVN 的内侧大细胞部和 SON 的背侧部，而 AVP-IR 神经元主要分布于 PVN 的大细胞部和 SON 的腹侧部。在 RWIS 大鼠中，PVN 中的 31%OT-IR 神经元和 40%AVP-IR 神经元中存在 Fos-IR 神经元，而在 SON 中分别代表 28%和 53%；(3)V(1b)R-IR 和 OTR-IR 神经元占据了 NTS 和 DMV 的所有部位。在 RWIS 大鼠中，DMV 中超过 10%的 OTR-IR 和 V(1b)R-IR 神经元被激活，而 NTS 中的比例较低。
RWIS 激活了 PVN 和 SON 中的催产素能和加压素能神经元，这些神经元可能通过 OTR 和 V(1b)R 投射到 NTS 或 DMV 中，从而调节神经元的活动。
