• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

胆固醇含量的多不饱和脂质膜的结构和动力学研究通过中子衍射和 NMR。

Structure and dynamics of cholesterol-containing polyunsaturated lipid membranes studied by neutron diffraction and NMR.

机构信息

Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA.

出版信息

J Membr Biol. 2011 Jan;239(1-2):63-71. doi: 10.1007/s00232-010-9326-6. Epub 2010 Dec 14.

DOI:10.1007/s00232-010-9326-6
PMID:21161517
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3030944/
Abstract

A direct and quantitative analysis of the internal structure and dynamics of a polyunsaturated lipid bilayer composed of 1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine (18:0-22:6n3-PC) containing 29 mol% cholesterol was carried out by neutron diffraction, (2)H-NMR and (13)C-MAS NMR. Scattering length distribution functions of cholesterol segments as well as of the sn-1 and sn-2 hydrocarbon chains of 18:0-22:6n3-PC were obtained by conducting experiments with specifically deuterated cholesterol and lipids. Cholesterol orients parallel to the phospholipids, with the A-ring near the lipid glycerol and the terminal methyl groups 3 Å away from the bilayer center. Previously, we reported that the density of polyunsaturated docosahexaenoic acid (DHA, 22:6n3) chains was higher near the lipid-water interface. Addition of cholesterol partially redistributes DHA density from near the lipid-water interface to the center of the hydrocarbon region. Cholesterol raises chain-order parameters of both stearic acid and DHA chains. The fractional order increase for stearic acid methylene carbons C(8)-C(18) is larger, reflecting the redistribution of DHA chain density toward the bilayer center. The correlation times of DHA chain isomerization are short and mostly unperturbed by the presence of cholesterol. The uneven distribution of saturated and polyunsaturated chain densities and the cholesterol-induced balancing of chain distributions may have important implications for the function and integrity of membrane receptors, such as rhodopsin.

摘要

通过中子衍射、(2)H-NMR 和 (13)C-MAS NMR，对由 1-硬脂酰-2-二十二碳六烯酰-sn-甘油-3-磷酸胆碱（18:0-22:6n3-PC）组成的多不饱和脂质双层的内部结构和动力学进行了直接和定量分析，该脂质双层含有 29mol%胆固醇。通过对胆固醇和脂质进行特定氘化的实验，获得了胆固醇片段以及 sn-1 和 sn-2 烃链的散射长度分布函数。胆固醇平行于磷脂取向，A 环靠近脂质甘油，末端甲基距双层中心 3Å。以前，我们报道过多不饱和二十二碳六烯酸 (DHA，22:6n3) 链的密度在靠近脂水界面处较高。胆固醇的添加部分将 DHA 密度从靠近脂水界面重新分布到烃区的中心。胆固醇提高了硬脂酸和 DHA 链的链有序参数。硬脂酸亚甲基碳原子 C(8)-C(18)的分数阶增加更大，反映了 DHA 链密度向双层中心的重新分布。DHA 链异构化的相关时间短，且大部分不受胆固醇的影响。饱和和多不饱和链密度的不均匀分布以及胆固醇诱导的链分布平衡可能对膜受体（如视紫红质）的功能和完整性具有重要意义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a52/3030944/2b4ffc3d2d22/232_2010_9326_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a52/3030944/a0435cfb1f9d/232_2010_9326_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a52/3030944/bd02d8a0dbc3/232_2010_9326_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a52/3030944/043a4088f3bc/232_2010_9326_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a52/3030944/58a319fd551d/232_2010_9326_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a52/3030944/2b4ffc3d2d22/232_2010_9326_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a52/3030944/a0435cfb1f9d/232_2010_9326_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a52/3030944/bd02d8a0dbc3/232_2010_9326_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a52/3030944/043a4088f3bc/232_2010_9326_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a52/3030944/58a319fd551d/232_2010_9326_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a52/3030944/2b4ffc3d2d22/232_2010_9326_Fig5_HTML.jpg

相似文献

1
Structure and dynamics of cholesterol-containing polyunsaturated lipid membranes studied by neutron diffraction and NMR.胆固醇含量的多不饱和脂质膜的结构和动力学研究通过中子衍射和 NMR。
J Membr Biol. 2011 Jan;239(1-2):63-71. doi: 10.1007/s00232-010-9326-6. Epub 2010 Dec 14.
2
Polyunsaturated docosahexaenoic vs docosapentaenoic acid-differences in lipid matrix properties from the loss of one double bond.多不饱和二十二碳六烯酸与二十二碳五烯酸——因一个双键缺失导致的脂质基质特性差异
J Am Chem Soc. 2003 May 28;125(21):6409-21. doi: 10.1021/ja029029o.
3
The structure of polyunsaturated lipid bilayers important for rhodopsin function: a neutron diffraction study.对视紫红质功能重要的多不饱和脂质双层结构：一项中子衍射研究。
Biophys J. 2006 Jan 1;90(1):L04-6. doi: 10.1529/biophysj.105.071712. Epub 2005 Oct 28.
4
Structure of docosahexaenoic acid-containing phospholipid bilayers as studied by (2)H NMR and molecular dynamics simulations.通过（2）H NMR和分子动力学模拟研究的含二十二碳六烯酸磷脂双层结构
J Am Chem Soc. 2002 Jan 16;124(2):298-309. doi: 10.1021/ja011383j.
5
Influence of docosahexaenoic acid and cholesterol on lateral lipid organization in phospholipid mixtures.二十二碳六烯酸和胆固醇对磷脂混合物中侧向脂质组织的影响。
Biochemistry. 1998 Dec 8;37(49):17299-308. doi: 10.1021/bi980078g.
6
Structural properties of docosahexaenoyl phospholipid bilayers investigated by solid-state 2H NMR spectroscopy.通过固态2H核磁共振光谱研究二十二碳六烯酰磷脂双层的结构特性。
J Am Chem Soc. 2001 Dec 19;123(50):12611-22. doi: 10.1021/ja011745n.
7
Packing and viscoelasticity of polyunsaturated omega-3 and omega-6 lipid bilayers as seen by (2)H NMR and X-ray diffraction.通过（2）H核磁共振和X射线衍射观察到的多不饱和ω-3和ω-6脂质双层的堆积和粘弹性
J Am Chem Soc. 2005 Feb 9;127(5):1576-88. doi: 10.1021/ja046453b.
8
Comparison of phosphatidylcholines containing one or two docosahexaenoic acyl chains on properties of phospholipid monolayers and bilayers.含一个或两个二十二碳六烯酸酰基链的磷脂酰胆碱对磷脂单分子层和双分子层性质的比较。
Biochim Biophys Acta. 1995 Jun 14;1236(2):266-72. doi: 10.1016/0005-2736(95)00058-b.
9
Cholesterol hydroxyl group is found to reside in the center of a polyunsaturated lipid membrane.胆固醇羟基位于多不饱和脂质膜的中心。
Biochemistry. 2006 Jan 31;45(4):1227-33. doi: 10.1021/bi0520840.
10
Molecular-level organization of saturated and polyunsaturated fatty acids in a phosphatidylcholine bilayer containing cholesterol.含胆固醇的磷脂酰胆碱双分子层中饱和脂肪酸和多不饱和脂肪酸的分子水平组织
Biochemistry. 2004 Dec 14;43(49):15318-28. doi: 10.1021/bi048231w.

引用本文的文献

1
Special issue for Klaus Gawrisch.献给克劳斯·高里施的特刊。
Biophys J. 2023 Mar 21;122(6):E1-E8. doi: 10.1016/j.bpj.2023.02.022. Epub 2023 Mar 15.
2
Order vs. Disorder: Cholesterol and Omega-3 Phospholipids Determine Biomembrane Organization.有序与无序：胆固醇和欧米伽-3 磷脂决定生物膜的组织。
Int J Mol Sci. 2022 May 10;23(10):5322. doi: 10.3390/ijms23105322.
3
All n-3 PUFA are not the same: MD simulations reveal differences in membrane organization for EPA, DHA and DPA.并非所有 n-3 PUFA 都一样：MD 模拟揭示了 EPA、DHA 和 DPA 在膜组织中的差异。

本文引用的文献

1
AND/R: Advanced neutron diffractometer/reflectometer for investigation of thin films and multilayers for the life sciences.AND/R：用于生命科学领域薄膜和多层膜研究的先进中子衍射仪/反射仪。
Rev Sci Instrum. 2006 Jul;77(7):74301-7430111. doi: 10.1063/1.2219744.
2
Purification of recombinant G-protein-coupled receptors.重组G蛋白偶联受体的纯化
Methods Enzymol. 2009;463:631-45. doi: 10.1016/S0076-6879(09)63036-6.
3
Cholesterol is found to reside in the center of a polyunsaturated lipid membrane.胆固醇存在于多不饱和脂质膜的中心。
Biochim Biophys Acta Biomembr. 2018 May;1860(5):1125-1134. doi: 10.1016/j.bbamem.2018.01.002. Epub 2018 Jan 3.
4
Membrane-Active Properties of an Amphitropic Peptide from the CyaA Toxin Translocation Region.两亲性肽的膜活性特性来自 CyaA 毒素转位区。
Toxins (Basel). 2017 Nov 14;9(11):369. doi: 10.3390/toxins9110369.
5
Characterization of lipid rafts in human platelets using nuclear magnetic resonance: A pilot study.利用核磁共振对人血小板中脂筏的表征：一项初步研究。
Biochem Biophys Rep. 2017 Mar 20;10:132-136. doi: 10.1016/j.bbrep.2017.03.005. eCollection 2017 Jul.
6
α-Tocopherol Is Well Designed to Protect Polyunsaturated Phospholipids: MD Simulations.α-生育酚旨在保护多不饱和磷脂：分子动力学模拟
Biophys J. 2015 Oct 20;109(8):1608-18. doi: 10.1016/j.bpj.2015.08.032.
7
n-3 polyunsaturated fatty acids suppress CD4(+) T cell proliferation by altering phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2] organization.n-3多不饱和脂肪酸通过改变磷脂酰肌醇-（4,5）-二磷酸[PI(4,5)P2]的组织来抑制CD4(+) T细胞增殖。
Biochim Biophys Acta. 2016 Jan;1858(1):85-96. doi: 10.1016/j.bbamem.2015.10.009. Epub 2015 Oct 23.
8
Cholesterol enhances surface water diffusion of phospholipid bilayers.胆固醇可增强磷脂双层膜的表面水扩散。
J Chem Phys. 2014 Dec 14;141(22):22D513. doi: 10.1063/1.4897539.
9
Membrane-sensitive conformational states of helix 8 in the metabotropic Glu2 receptor, a class C GPCR.代谢型谷氨酸受体 2 中螺旋 8 的膜敏感构象状态，一种 C 类 GPCR。
PLoS One. 2012;7(8):e42023. doi: 10.1371/journal.pone.0042023. Epub 2012 Aug 1.
10
Docosahexaenoic and eicosapentaenoic acids segregate differently between raft and nonraft domains.二十二碳六烯酸和二十碳五烯酸在筏和非筏域之间的分离不同。
Biophys J. 2012 Jul 18;103(2):228-37. doi: 10.1016/j.bpj.2012.06.016. Epub 2012 Jul 17.
Biochemistry. 2008 Jul 8;47(27):7090-6. doi: 10.1021/bi800123b. Epub 2008 Jun 11.
4
Regulation of membrane proteins by dietary lipids: effects of cholesterol and docosahexaenoic acid acyl chain-containing phospholipids on rhodopsin stability and function.膳食脂质对膜蛋白的调节作用：胆固醇和含二十二碳六烯酸酰基链的磷脂对视紫红质稳定性和功能的影响。
Biophys J. 2008 Aug;95(3):1206-16. doi: 10.1529/biophysj.107.122788. Epub 2008 Apr 18.
5
Docosahexaenoyl chains isomerize on the sub-nanosecond time scale.二十二碳六烯酰链在亚纳秒时间尺度上发生异构化。
J Am Chem Soc. 2007 May 30;129(21):6678-9. doi: 10.1021/ja068856c. Epub 2007 May 4.
6
Evidence for specificity in lipid-rhodopsin interactions.脂质-视紫红质相互作用特异性的证据。
J Biol Chem. 2006 Nov 3;281(44):33233-41. doi: 10.1074/jbc.M603059200. Epub 2006 Sep 7.
7
The structure of polyunsaturated lipid bilayers important for rhodopsin function: a neutron diffraction study.对视紫红质功能重要的多不饱和脂质双层结构：一项中子衍射研究。
Biophys J. 2006 Jan 1;90(1):L04-6. doi: 10.1529/biophysj.105.071712. Epub 2005 Oct 28.
8
Molecular-level organization of saturated and polyunsaturated fatty acids in a phosphatidylcholine bilayer containing cholesterol.含胆固醇的磷脂酰胆碱双分子层中饱和脂肪酸和多不饱和脂肪酸的分子水平组织
Biochemistry. 2004 Dec 14;43(49):15318-28. doi: 10.1021/bi048231w.
9
Understanding sterol-membrane interactions, part II: complete 1H and 13C assignments by solid-state NMR spectroscopy and determination of the hydrogen-bonding partners of cholesterol in a lipid bilayer.了解甾醇与膜的相互作用，第二部分：通过固态核磁共振光谱法完成1H和13C归属以及确定脂质双层中胆固醇的氢键结合伙伴
Chemistry. 2004 Nov 19;10(23):6005-14. doi: 10.1002/chem.200400246.
10
Polyunsaturated docosahexaenoic vs docosapentaenoic acid-differences in lipid matrix properties from the loss of one double bond.多不饱和二十二碳六烯酸与二十二碳五烯酸——因一个双键缺失导致的脂质基质特性差异
J Am Chem Soc. 2003 May 28;125(21):6409-21. doi: 10.1021/ja029029o.