脑益康对谷氨酸诱导的海马神经元损伤的保护作用
[Protective effect of Naoyikang on the damage induced by glutamate in hippocampal neuron].
作者信息
Hu Ya-E, Zhou Ai-Ling, Zhu Yan, Mao Jia-Hui, Shi Hai-Yan
机构信息
Department of Pathophysiology, Basic Medical College of Nantong University, Nantong 226001, China.
出版信息
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2007 Aug;23(3):300-3.
AIM
To investigate the effect of Naoyikang serum on the damage induced by glutamate in hippocampal neuron.
METHODS
Morphological observation, MTT assay and nuclear DNA-associated fluorescence with DAPI dye were applied to evaluate the viability of hippocampal neuron, immunocytochemistry and RT-PCR were used to determine the expression of PTEN.
RESULTS
A decreased viability and increased expression of PTEN were shown in hippocampal neuron in response to the treatment with glutamate. It was shown that the percentage of cell death and the expression of PTEN were reduced by the treatment with Naoyikang serum.
CONCLUSION
These results suggest that Naoyikang may prevent the toxicity of glutamate by suppressing the expression of PTEN.
目的
研究脑益康血清对谷氨酸诱导的海马神经元损伤的影响。
方法
采用形态学观察、MTT 法以及用 DAPI 染料进行核 DNA 相关荧光检测来评估海马神经元的活力，运用免疫细胞化学和 RT-PCR 法检测 PTEN 的表达。
结果
谷氨酸处理后海马神经元活力降低，PTEN 表达增加。脑益康血清处理可降低细胞死亡率及 PTEN 的表达。
结论
这些结果表明脑益康可能通过抑制 PTEN 的表达来预防谷氨酸的毒性。
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