人参皂苷Rb3对大鼠海马培养神经元谷氨酸兴奋性毒性损伤的影响及机制
[Effects and mechanisms of ginsenoside Rb3 on glutamate excitotoxic injury in cultured neurons of rat hippocampus].
作者信息
Shen Hong-Mei, Jiang Zheng-Lin, Gu Xiao-Song
机构信息
Institute of Nautical Medicine, Nantong University, Nantong 226001, China.
出版信息
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2006 Feb;22(1):31-4.
AIM
To observe protective effects of ginsenoside Rb3 on glutamate excitotoxic injury in cultured hippocampal neurons and involved mechanisms.
METHODS
On cultured rat hippocampal neurons treated with glutamate at toxic concentration, we made the following investigations: by using MTT assay, LDH leakage detection, tests of total NOS, iNOS and cNOS activity, and the protective effects of ginsenoside Rb3.
RESULTS
Ginsenoside Rb3 can enhance the hippocampal neuronal viability, decrease the LDH leakage, elevate the viability of cNOS, and in the same time weaken iNOS's viability.
CONCLUSION
Ginsenoside Rb3 has the significant protective effects on glutamate excitotoxic injury. The involved mechanism may include antagonizing the injury of neuron membrane, inhibiting the viability of iNOS, and increasing the activity of cNOS.
目的
观察人参皂苷Rb3对培养海马神经元谷氨酸兴奋性毒性损伤的保护作用及其相关机制。
方法
用毒性浓度的谷氨酸处理培养的大鼠海马神经元，进行以下研究：采用MTT法、检测乳酸脱氢酶（LDH）漏出率、检测总一氧化氮合酶（NOS）、诱导型NOS（iNOS）和组成型NOS（cNOS）活性，以及人参皂苷Rb3的保护作用。
结果
人参皂苷Rb3可提高海马神经元活力，降低LDH漏出率，提高cNOS活力，同时减弱iNOS活力。
结论
人参皂苷Rb3对谷氨酸兴奋性毒性损伤具有显著保护作用。其相关机制可能包括拮抗神经元膜损伤、抑制iNOS活力及增加cNOS活性。
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