University of Medicine and Pharmacy, Carol Davila, Eroii Sanitari Bulevard 8, Bucharest, Romania.
BMC Microbiol. 2010 Dec 17;10:322. doi: 10.1186/1471-2180-10-322.
A microcalorimetric study was carried out using a Staphylococcus epidermidis population to determine the reproducibility of bacterial growth and the variability of the results within certain experimental parameters (temperature, bacterial concentration, sample thermal history). Reproducibility tests were performed as series of experiments within the same conditions using either freshly prepared populations or samples kept in cold storage. In both cases, the samples were obtained by serial dilution from a concentrated TSB bacterial inoculum incubated overnight.
The results show that experiments are fairly reproducible and that specimens can be preserved at low temperatures (1 - 2°C) at least 4 days. The thermal signal variations at different temperatures and initial bacterial concentrations obey a set of rules that we identified.
Our study adds to the accumulating data and confirms available results of isothermal microcalorimetry applications in microbiology and can be used to standardize this method for either research or clinical setting.
本研究采用表皮葡萄球菌种群进行微量量热法研究，以确定细菌生长的重现性和特定实验参数（温度、细菌浓度、样品热历史）内结果的可变性。重现性测试是在相同条件下进行的一系列实验，使用新鲜制备的种群或冷藏保存的样品。在这两种情况下，样品均通过从过夜孵育的浓缩 TSB 细菌接种物中进行连续稀释获得。
结果表明，实验具有相当的重现性，并且标本可以在低温（1-2°C）下保存至少 4 天。在不同温度和初始细菌浓度下的热信号变化遵循我们确定的一组规则。
我们的研究增加了积累的数据，并证实了等温微量量热法在微生物学中的应用的现有结果，并且可以用于为研究或临床环境标准化该方法。
