微量量热法用于金黄色葡萄球菌临床分离株中耐甲氧西林早期检测的性能
Performance of microcalorimetry for early detection of methicillin resistance in clinical isolates of Staphylococcus aureus.
作者信息
Baldoni Daniela, Hermann Heinz, Frei Reno, Trampuz Andrej, Steinhuber Andrea
机构信息
Infectious Diseases Research Laboratory, Department of Biomedicine, University Hospital, Basel, Switzerland.
出版信息
J Clin Microbiol. 2009 Mar;47(3):774-6. doi: 10.1128/JCM.02374-08. Epub 2009 Jan 21.
Abstract
We describe a calorimetric assay for the detection of methicillin-resistant Staphylococcus aureus (MRSA) within 5 h. Microbial heat was calculated in culture with and without cefoxitin. Among 30 genetically distinct clinical isolates, 19/20 MRSA (95%) and 10/10 methicillin-susceptible Staphylococcus aureus (100%) were correctly identified. Microcalorimetry may be useful for rapid MRSA screening.
摘要
我们描述了一种在5小时内检测耐甲氧西林金黄色葡萄球菌（MRSA）的量热测定法。在有和没有头孢西丁的培养物中计算微生物产热。在30株基因不同的临床分离株中，19/20株MRSA（95%）和10/10株甲氧西林敏感金黄色葡萄球菌（100%）被正确鉴定。微量量热法可能有助于快速筛查MRSA。
相似文献
1
Performance of microcalorimetry for early detection of methicillin resistance in clinical isolates of Staphylococcus aureus.微量量热法用于金黄色葡萄球菌临床分离株中耐甲氧西林早期检测的性能
J Clin Microbiol. 2009 Mar;47(3):774-6. doi: 10.1128/JCM.02374-08. Epub 2009 Jan 21.
2
Rapid differentiation of methicillin-susceptible Staphylococcus aureus from methicillin-resistant S. aureus and MIC determinations by isothermal microcalorimetry.通过等温微量热法快速区分甲氧西林敏感金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌并测定最低抑菌浓度
J Clin Microbiol. 2008 Jun;46(6):2083-7. doi: 10.1128/JCM.00611-08. Epub 2008 Apr 16.
3
Rapid differentiation of methicillin-resistant Staphylococcus aureus and methicillin-susceptible Staphylococcus aureus from blood cultures by use of a direct cefoxitin disk diffusion test.通过直接头孢西丁纸片扩散试验快速从血培养物中鉴别耐甲氧西林金黄色葡萄球菌和甲氧西林敏感金黄色葡萄球菌
J Clin Microbiol. 2008 Nov;46(11):3836-8. doi: 10.1128/JCM.01276-08. Epub 2008 Oct 1.
4
Evaluation of a cefoxitin disk diffusion test for the routine detection of methicillin-resistant Staphylococcus aureus.用于常规检测耐甲氧西林金黄色葡萄球菌的头孢西丁纸片扩散试验的评估
Clin Microbiol Infect. 2004 Aug;10(8):762-5. doi: 10.1111/j.1469-0691.2004.00919.x.
5
Evaluation of three techniques for detection of low-level methicillin-resistant Staphylococcus aureus (MRSA): a disk diffusion method with cefoxitin and moxalactam, the Vitek 2 system, and the MRSA-screen latex agglutination test.三种检测低水平耐甲氧西林金黄色葡萄球菌（MRSA）技术的评估：使用头孢西丁和莫拉西林的纸片扩散法、Vitek 2系统以及MRSA筛查乳胶凝集试验。
J Clin Microbiol. 2002 Aug;40(8):2766-71. doi: 10.1128/JCM.40.8.2766-2771.2002.
6
Evaluation of a new chromogenic medium, MRSA select, for detection of methicillin-resistant Staphylococcus aureus.评估一种新型显色培养基MRSA select用于检测耐甲氧西林金黄色葡萄球菌的效果。
J Clin Microbiol. 2006 Dec;44(12):4561-3. doi: 10.1128/JCM.01763-06. Epub 2006 Oct 18.
7
Evaluation of new Vitek 2 card and disk diffusion method for determining susceptibility of Staphylococcus aureus to oxacillin.评估新型Vitek 2卡片和纸片扩散法测定金黄色葡萄球菌对苯唑西林敏感性的效果。
J Clin Microbiol. 2008 Aug;46(8):2525-8. doi: 10.1128/JCM.00291-08. Epub 2008 Jun 11.
8
Evaluation of mannitol salt agar, CHROMagar Staph aureus and CHROMagar MRSA for detection of meticillin-resistant Staphylococcus aureus from nasal swab specimens.评估甘露醇盐琼脂、CHROMagar金黄色葡萄球菌培养基和CHROMagar耐甲氧西林金黄色葡萄球菌培养基用于从鼻拭子标本中检测耐甲氧西林金黄色葡萄球菌的效果。
J Med Microbiol. 2007 Jan;56(Pt 1):43-46. doi: 10.1099/jmm.0.46777-0.
9
Comparison of Different Phenotypic Approaches To Screen and Detect -Harboring Methicillin-Resistant Staphylococcus aureus.比较不同表型方法以筛查和检测耐甲氧西林金黄色葡萄球菌。
J Clin Microbiol. 2017 Dec 26;56(1). doi: 10.1128/JCM.00826-17. Print 2018 Jan.
10
Rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-susceptible Staphylococcus aureus (MSSA) using the KeyPath MRSA/MSSA blood culture test and the BacT/ALERT system in a pediatric population.采用 KeyPath MRSA/MSSA 血培养检测和 BacT/ALERT 系统快速检测儿童人群中的耐甲氧西林金黄色葡萄球菌（MRSA）和甲氧西林敏感金黄色葡萄球菌（MSSA）。
Arch Pathol Lab Med. 2013 Aug;137(8):1103-5. doi: 10.5858/arpa.2012-0422-OA.
引用本文的文献
1
Assessment of Arthrobacter oxydans Subcellular Structural Stability in Response to Metal Action using Differential Scanning Calorimetry.使用差示扫描量热法评估氧化节杆菌亚细胞结构稳定性对金属作用的响应。
Cell Biochem Biophys. 2025 Feb 23. doi: 10.1007/s12013-025-01688-4.
2
Rapid and Simple Morphological Assay for Determination of Susceptibility/Resistance to Combined Ciprofloxacin and Ampicillin, Independently, in .用于独立测定对环丙沙星和氨苄西林联合药敏/耐药性的快速简便形态学检测法
Antibiotics (Basel). 2024 Jul 20;13(7):676. doi: 10.3390/antibiotics13070676.
3
Evolution of Antibacterial Drug Screening Methods: Current Prospects for Mycobacteria.抗菌药物筛选方法的演变：分枝杆菌的当前前景
Microorganisms. 2021 Dec 10;9(12):2562. doi: 10.3390/microorganisms9122562.
4
Detection and Drug Susceptibility Testing of Using Isothermal Microcalorimetry.使用等温微量热法进行检测及药敏试验。
Microorganisms. 2021 Nov 11;9(11):2337. doi: 10.3390/microorganisms9112337.
5
Photocatalytic Nanowires-Based Air Filter: Towards Reusable Protective Masks.基于光催化纳米线的空气过滤器：迈向可重复使用的防护口罩。
Adv Funct Mater. 2020 Oct 1;30(40):2004615. doi: 10.1002/adfm.202004615. Epub 2020 Aug 7.
6
Sub-nanowatt microfluidic single-cell calorimetry.亚毫微微升微流控单细胞量热法。
Nat Commun. 2020 Jun 12;11(1):2982. doi: 10.1038/s41467-020-16697-5.
7
In vitro antimicrobial susceptibility testing methods: agar dilution to 3D tissue-engineered models.体外抗菌药敏试验方法：琼脂稀释法至 3D 组织工程模型。
Eur J Clin Microbiol Infect Dis. 2018 Feb;37(2):187-208. doi: 10.1007/s10096-017-3089-2. Epub 2017 Sep 4.
8
Evaluation of the Antibacterial Effects of Flavonoid Combination from the Leaves of by Microcalorimetry and the Quadratic Rotary Combination Design.用微量量热法和二次旋转组合设计评价[植物名称]叶中黄酮类化合物组合的抗菌效果 。
注：原文中“from the Leaves of ”后面缺少具体植物名称。
Front Pharmacol. 2017 Feb 17;8:70. doi: 10.3389/fphar.2017.00070. eCollection 2017.
9
Isothermal microcalorimetry accurately detects bacteria, tumorous microtissues, and parasitic worms in a label-free well-plate assay.等温微量热法可在无标记微孔板检测中准确检测细菌、肿瘤微组织和寄生虫。
Biotechnol J. 2015 Mar;10(3):460-8. doi: 10.1002/biot.201400494. Epub 2015 Feb 18.
10
The potential use of microcalorimetry in rapid differentiation between septic arthritis and other causes of arthritis.微量量热法在快速鉴别化脓性关节炎与其他关节炎病因方面的潜在应用。
Eur J Clin Microbiol Infect Dis. 2015 Mar;34(3):461-5. doi: 10.1007/s10096-014-2248-y. Epub 2014 Sep 25.
本文引用的文献
1
Rapid differentiation of methicillin-susceptible Staphylococcus aureus from methicillin-resistant S. aureus and MIC determinations by isothermal microcalorimetry.通过等温微量热法快速区分甲氧西林敏感金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌并测定最低抑菌浓度
J Clin Microbiol. 2008 Jun;46(6):2083-7. doi: 10.1128/JCM.00611-08. Epub 2008 Apr 16.
2
Microbiological evaluation of a new growth-based approach for rapid detection of methicillin-resistant Staphylococcus aureus.一种基于生长的新型方法用于快速检测耐甲氧西林金黄色葡萄球菌的微生物学评估
J Antimicrob Chemother. 2008 Jun;61(6):1277-80. doi: 10.1093/jac/dkn122. Epub 2008 Mar 20.
3
Use of cefoxitin-based selective broth for improved detection of methicillin-resistant Staphylococcus aureus.使用基于头孢西丁的选择性肉汤改良耐甲氧西林金黄色葡萄球菌的检测方法。
J Clin Microbiol. 2006 Feb;44(2):592-4. doi: 10.1128/JCM.44.2.592-594.2006.
4
Guidelines for the laboratory diagnosis and susceptibility testing of methicillin-resistant Staphylococcus aureus (MRSA).耐甲氧西林金黄色葡萄球菌（MRSA）实验室诊断及药敏试验指南。
J Antimicrob Chemother. 2005 Dec;56(6):1000-18. doi: 10.1093/jac/dki372. Epub 2005 Nov 17.
5
Comprehensive strategy to prevent nosocomial spread of methicillin-resistant Staphylococcus aureus in a highly endemic setting.在高流行环境中预防耐甲氧西林金黄色葡萄球菌医院内传播的综合策略。
Arch Intern Med. 2004 Oct 11;164(18):2038-43. doi: 10.1001/archinte.164.18.2038.
6
Molecular typing of methicillin-resistant Staphylococcus aureus: can PCR replace pulsed-field gel electrophoresis?耐甲氧西林金黄色葡萄球菌的分子分型：聚合酶链反应能否取代脉冲场凝胶电泳？
J Clin Microbiol. 2003 Jul;41(7):3181-6. doi: 10.1128/JCM.41.7.3181-3186.2003.
7
Comparison of culture screening methods for detection of nasal carriage of methicillin-resistant Staphylococcus aureus: a prospective study comparing 32 methods.检测耐甲氧西林金黄色葡萄球菌鼻腔定植的培养筛查方法比较：一项比较32种方法的前瞻性研究
J Clin Microbiol. 2003 Jul;41(7):3163-6. doi: 10.1128/JCM.41.7.3163-3166.2003.
8
Evaluation of three techniques for detection of low-level methicillin-resistant Staphylococcus aureus (MRSA): a disk diffusion method with cefoxitin and moxalactam, the Vitek 2 system, and the MRSA-screen latex agglutination test.三种检测低水平耐甲氧西林金黄色葡萄球菌（MRSA）技术的评估：使用头孢西丁和莫拉西林的纸片扩散法、Vitek 2系统以及MRSA筛查乳胶凝集试验。
J Clin Microbiol. 2002 Aug;40(8):2766-71. doi: 10.1128/JCM.40.8.2766-2771.2002.
9
Role of mecA transcriptional regulation in the phenotypic expression of methicillin resistance in Staphylococcus aureus.mecA转录调控在金黄色葡萄球菌耐甲氧西林表型表达中的作用
J Bacteriol. 1996 Sep;178(18):5464-71. doi: 10.1128/jb.178.18.5464-5471.1996.
10
Evidence that the National Committee for Clinical Laboratory Standards disk test is less sensitive than the screen plate for detection of low-expression-class methicillin-resistant Staphylococcus aureus.有证据表明，在检测低表达型耐甲氧西林金黄色葡萄球菌方面，美国国家临床实验室标准委员会纸片扩散法比平皿筛选法敏感性更低。
J Clin Microbiol. 1995 Jul;33(7):1909-11. doi: 10.1128/jcm.33.7.1909-1911.1995.
Zotero 插件