Zhong Shu-Ling, Feng Yan, Xu Chang-Ping, Xu Qi, Lu Yi-Yu
Zhejiang Provincial Center for Disease Control and Prevention, Key Laboratory of Emergence Detection for Public Health of Zhejiang Province, Hangzhou 310051, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2010 Aug;31(8):909-12.
To investigate the genetic characteristics and variation within the phosphoprotein (P) gene of measles epidemic strains circulated in Zhejiang province.
The whole sequence of P gene of the epidemic strains related to Zhejiang Measles virus during 1999 to 2008 was amplified, using the RT-PCR Assay. PCR products were sequenced and compared with the sequences of measles vaccine and other epidemic strains.
Totally, 1524 nucleotides were sequenced from each epidemic strain and 507 amino acids were derived correspondingly. Compared with the vaccine strain, there were 59 - 75 nucleotides (divergent ratios were 3.9% - 4.9%) mutated from the epidemic strains, which were isolated during 1999 to 2008 and causing mutation on 36 - 42 amino acid (divergent ratios were 7.1% - 8.3%). Changes were also observed on the secondary structure. The phylogenetic tree, constructed based on the sequences of P gene, was similar to that based on the N gene, recommended by WHO. In addition, the average divergent ratio of P protein was greater than the ratio occurred on the N and H genes.
The variation within the P gene between the vaccine and epidemic strains circulated in Zhejiang province during 1999 to 2008 was significant.
研究浙江省流行的麻疹病毒株磷蛋白（P）基因的遗传特征及变异情况。
采用RT-PCR法扩增1999年至2008年浙江省麻疹病毒流行株P基因的全长序列，对PCR产物进行测序，并与麻疹疫苗株及其他流行株的序列进行比较。
每株流行株共测序1524个核苷酸，相应推导507个氨基酸。与疫苗株相比，1999年至2008年分离的流行株有59 - 75个核苷酸发生突变（差异率为3.9% - 4.9%），导致36 - 42个氨基酸发生突变（差异率为7.1% - 8.3%），二级结构也有变化。基于P基因序列构建的系统发育树与WHO推荐的基于N基因构建的系统发育树相似。此外，P蛋白的平均差异率大于N基因和H基因。
1999年至2008年浙江省流行的疫苗株与麻疹病毒流行株之间P基因变异明显。
