Zhang Dong-Yan, Feng Yan, Zhong Shu-Ling, Lu Yi-Yu, Zhuang Fang-Cheng, Xu Chang-Ping
Department of Epidemiology and Health Statistics of Medical School of Ningbo University, Ningbo 315211, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2012 Mar;46(3):252-7.
To compare the differences in the complete genome sequence between mumps epidemic strain and mumps vaccine strain S79 isolated in Zhejiang province.
A total of 4 mumps epidemic strains, which were separated from Zhejiang province during 2005 to 2010, named as ZJ05-1, ZJ06-3, ZJ08-1 and ZJ10-1 were selected in the study. The complete genome sequences were amplified using RT-PCR. The genetic differences between vaccine strain S79 and other genotype strains were compared; while the genetic-distance was calculated and the evolution was analyzed.
The biggest difference between the 4 epidemic strains and the vaccine strain S79 was found on the membrane associated protein gene; whose average nucleotide differential number was 42.5 +/- 3.0 and the average variant ratio was 13.6%; while the mean amino acid differential number was 12.8 +/- 1.5 and the average variant ratio was 22.4%. The smallest difference among the 4 epidemic strains and the vaccine strain was found in stromatin genes, whose average nucleotide differential number was 73.8 +/- 2.5 and the average variant ratio was 5.9%; while the mean amino acid differential number was 3.0 +/- 0.8 and the average variant ratio was 0.8%. The dn/ds value of the stromatin genes of the 4 epidemic strains reached the highest, as 0.6526; but without any positive pressure (dn/ds < 1, chi2 = 0.87, P > 0.05). There were mutations happened on the known antigen epitope, as 8th amino acid of membrane associated protein genes and on the 336th and 356th amino acid of hemagglutinin/neuraminidase proteins. Compared with the vaccine strain, the glycosylation sites of ZJ05-1, ZJ06-3, ZJ08-1 and ZJ10-1 increased 1, 1, 2 and 2 respectively. The complete amino acid sequence of all strains showed that there were 17 characteristic sites found on the genotype-F mumps strain. Within the complete genome, the genetic-distance between epidemic strains and vaccine strains in Zhejiang province (0.071) was significantly larger than the genetic-distance between strains in Yunnan province (0.013); the difference showing statistical significance (t = 4.14, P < 0.05). Except nucleocapsid protein genes, all the genes shared similar evolution tree.
There were significant differences found in the genes between mumps epidemic strain and mumps vaccine in Zhejiang province.
比较流行性腮腺炎流行株与浙江省分离的腮腺炎疫苗株S79全基因组序列的差异。
选取2005年至2010年期间从浙江省分离的4株流行性腮腺炎流行株，命名为ZJ05 - 1、ZJ06 - 3、ZJ08 - 1和ZJ10 - 1。采用RT - PCR扩增全基因组序列。比较疫苗株S79与其他基因型株之间的遗传差异；计算遗传距离并进行进化分析。
4株流行株与疫苗株S79之间差异最大的是膜相关蛋白基因；其平均核苷酸差异数为42.5±3.0，平均变异率为13.6%；平均氨基酸差异数为12.8±1.5，平均变异率为22.4%。4株流行株与疫苗株之间差异最小的是基质蛋白基因，其平均核苷酸差异数为73.8±2.5，平均变异率为5.9%；平均氨基酸差异数为3.0±0.8，平均变异率为0.8%。4株流行株基质蛋白基因的dn/ds值最高，为0.6526；但无正选择压力（dn/ds < 1，χ2 = 0.87，P > 0.05）。已知抗原表位发生了突变，如膜相关蛋白基因的第8位氨基酸以及血凝素/神经氨酸酶蛋白的第336位和第356位氨基酸。与疫苗株相比，ZJ05 - 1、ZJ06 - 3、ZJ08 - 1和ZJ10 - 1的糖基化位点分别增加了1个、1个、2个和2个。所有毒株的完整氨基酸序列显示，在F基因型腮腺炎毒株上发现了17个特征位点。在全基因组内，浙江省流行株与疫苗株之间的遗传距离（0.071）显著大于云南省毒株之间的遗传距离（0.013）；差异具有统计学意义（t = 4.14，P < 0.05）。除核衣壳蛋白基因外，所有基因的进化树相似。
浙江省流行性腮腺炎流行株与腮腺炎疫苗株在基因上存在显著差异。
