Gong Li-Ming, Ge Qiong, Chen Yin, Lu Yi-Yu, Zhang Yan-Jun, Yan Ju-Ying, Zhou Min, Shi Wen
Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2011 May;32(5):490-3.
To study the molecular characteristic of norovirus in 3 outbreaks of gastroenteritis in Zhejiang province.
During January 2008 and December 2009, fecal specimens of patients were collected from 3 outbreaks of acute viral gastroenteritis. Noroviruses were detected by Real-time RT-PCR. Part of the positive samples were randomly selected and detected by RT-PCR. PCR products were sequenced. Sequence analysis was undertaken based on partial sequence of RNA dependent RNA polymerase (RdRp) and capsid protein gene. Some positive samples were amplified by 3'RACE (rapid amplification of cDNA 3'ends), 3200 bp in length. The exact whole ORF2, ORF3 and 3'untranslation regions (UTR) gene of norovirus were identified.
There were in total 3 outbreaks of viral gastroenteritis caused by norovirus being reported. A total of 62 stools were obtained from cases with acute gastroenteritis. Noroviruses were detected in 41 cases including 27 strains of genogroup I norovirus and 9 strains of genogroup II norovirus, 5 strains of genogroup I + II norovirus. Four genotypes including GI.8, GII.b, GI.2/GI.6 recombination together with co-infection of GI.8 and GII.b were detected.
Norovirus was confirmed as the major cause of outbreaks of viral gastroenteritis in Zhejiang province and multiple genotype of norovirus were identified from the outbreaks. It was the first time to have found a recombinant of GI.6 capsid and GI.2 polymerase norovirus as well as the co-infection of GI.8 and GII.b norovirus in the same sample.
研究浙江省3起胃肠炎暴发中诺如病毒的分子特征。
2008年1月至2009年12月期间，从3起急性病毒性胃肠炎暴发中收集患者粪便标本。采用实时逆转录聚合酶链反应（Real-time RT-PCR）检测诺如病毒。随机选取部分阳性样本进行逆转录聚合酶链反应（RT-PCR）检测。对PCR产物进行测序。基于RNA依赖的RNA聚合酶（RdRp）和衣壳蛋白基因的部分序列进行序列分析。部分阳性样本通过3'端cDNA快速扩增（3'RACE）进行扩增，长度为3200 bp。鉴定出诺如病毒确切的完整开放阅读框2（ORF2）、开放阅读框3（ORF3）和3'非翻译区（UTR）基因。
共报告3起因诺如病毒引起的病毒性胃肠炎暴发。从急性胃肠炎病例中总共获得62份粪便。41例检测出诺如病毒，其中包括27株I基因组诺如病毒、9株II基因组诺如病毒、5株I + II基因组诺如病毒。检测到4种基因型，包括GI.8、GII.b、GI.2/GI.6重组型以及GI.8和GII.b的共同感染。
诺如病毒被确认为浙江省病毒性胃肠炎暴发的主要病因，且从暴发中鉴定出多种诺如病毒基因型。首次在同一样本中发现GI.6衣壳和GI.2聚合酶诺如病毒重组体以及GI.8和GII.b诺如病毒的共同感染。
