Molecular Vaccinology Laboratory, Queensland Institute of Medical Research, Brisbane, QLD 4006, Australia.
Vaccine. 2011 Feb 11;29(8):1538-44. doi: 10.1016/j.vaccine.2010.11.091. Epub 2010 Dec 14.
The heat-labile enterotoxin of Escherichia coli (LT) consists of an enzymatically active A subunit (LTA) and a pentameric B subunit (LTB). LT has been extensively studied as a potent modulator of immune responses but wild-type LT is toxic and therefore unsuitable for clinical use. Approaches pursued to avoid the toxicity associated with the use of the native toxin while retaining its adjuvant properties have included isolation of subunit B (LTB) and construction of non-toxic LT AB complex mutants, such as LTK63 mutant. Here we review the immunomodulatory characteristics of LTB and LTK63 and their potential as mucosal and parenteral vaccine adjuvants.
大肠杆菌不耐热肠毒素（LT）由一个酶活性的 A 亚单位（LTA）和一个五聚体的 B 亚单位（LTB）组成。LT 作为一种有效的免疫调节剂已经被广泛研究，但野生型 LT 是有毒的，因此不适合临床应用。为了避免使用天然毒素带来的毒性，同时保留其佐剂特性，人们采用了多种方法，包括分离 B 亚单位（LTB）和构建无毒 LT AB 复合物突变体，如 LTK63 突变体。在这里，我们回顾了 LTB 和 LTK63 的免疫调节特性及其作为黏膜和肌肉内疫苗佐剂的潜力。
