Massachusetts General Hospital/Harvard Medical School, 114 16th Street, Charlestown, MA 02129, USA.
Methods. 2011 Mar;53(3):201-7. doi: 10.1016/j.ymeth.2010.12.009. Epub 2010 Dec 14.
Aggregation of amyloid beta peptide into senile plaques and hyperphosphorylated tau protein into neurofibrillary tangles in the brain are the pathological hallmarks of Alzheimer's disease. Despite over a century of research into these lesions, the exact relationship between pathology and neurotoxicity has yet to be fully elucidated. In order to study the formation of plaques and tangles and their effects on the brain, we have applied multiphoton in vivo imaging of transgenic mouse models of Alzheimer's disease. This technique allows longitudinal imaging of pathological aggregation of proteins and the subsequent changes in surrounding neuropil neurodegeneration and recovery after therapeutic interventions.
淀粉样 β 肽聚集形成老年斑，过度磷酸化的 tau 蛋白聚集形成神经原纤维缠结，这是阿尔茨海默病的病理特征。尽管对这些病变进行了一个多世纪的研究，但病理与神经毒性的确切关系尚未完全阐明。为了研究斑块和缠结的形成及其对大脑的影响，我们应用了阿尔茨海默病转基因小鼠模型的多光子在体成像技术。该技术允许对蛋白质的病理性聚集进行纵向成像，以及随后对周围神经突神经退行性变的变化和治疗干预后的恢复进行成像。
