Aquatic Ecology and Toxicology Section, Department of Zoology, University of Heidelberg, Im Neuenheimer Feld 230; D-69120 Heidelberg, Germany.
Comp Biochem Physiol C Toxicol Pharmacol. 2011 Apr;153(3):318-27. doi: 10.1016/j.cbpc.2010.12.002. Epub 2010 Dec 14.
The zebrafish embryo test is a widely used bioassay for the testing of chemicals, effluents and other types of environmental samples. Oxygen depletion in the testing of sediments and effluents is especially important and may be a confounding factor in the interpretation of apparent toxicity. In order to identify oxygen levels critical to early developmental stages of zebrafish, oxygen consumption of zebrafish embryos between 0 and 96h post-fertilization, minimum oxygen levels required by the embryos for survival as well as the effects of oxygen depletion following exposure to model sediments were determined. No significant effects on zebrafish embryo development were observed for oxygen concentrations between 7.15 and 3.33mg/L, whereas at concentrations between 3.0and 2.0mg/L minor developmental retardations were observed, yet without any pathological consequences. Oxygen concentrations lower than 0.88mg/L were 100% lethal. In the sediment contact tests with zebrafish embryos, native sediments rich in organic materials rapidly developed strongly hypoxic conditions, particularly at the sediment-water interface (0 to 500μm distance to the sediment).
斑马鱼胚胎测试是一种广泛用于测试化学品、废水和其他类型环境样本的生物测定方法。在测试沉积物和废水时，氧气消耗尤其重要，并且可能是解释明显毒性的混杂因素。为了确定对斑马鱼早期发育阶段至关重要的氧气水平，确定了受精后 0 至 96 小时斑马鱼胚胎的耗氧量、胚胎存活所需的最低氧气水平以及暴露于模型沉积物后氧气消耗的影响。在 7.15 至 3.33mg/L 之间的氧气浓度下，对斑马鱼胚胎发育没有明显影响，而在 3.0 至 2.0mg/L 之间的氧气浓度下，观察到轻微的发育迟缓，但没有任何病理后果。氧气浓度低于 0.88mg/L 时，斑马鱼胚胎 100%致死。在斑马鱼胚胎的沉积物接触测试中，富含有机物质的天然沉积物迅速形成强烈的缺氧条件，特别是在沉积物-水界面（距沉积物 0 至 500μm 处）。
