Department of Bioengineering, Imperial College London, London SW7 2AZ, UK.
Biosens Bioelectron. 2011 Feb 15;26(6):2890-6. doi: 10.1016/j.bios.2010.11.033. Epub 2010 Dec 1.
We have developed a stable and selective ATP biosensor for long-term in vitro tissue monitoring. The electrode was fabricated by entrapping glucose oxidase (GOx) and hexokinase (HEX) in a poly-phenol film on a Pt microelectrode. The biosensor was stable to a fixed concentration of glucose for over 20 min and had a limit of detection of 9.9 ± 3.2 nM, with a sensitivity of 45.8 ± 1.22 pA μM(-1). Most significantly of all, the response on the ATP biosensor did not alter in the presence of 1mM ascorbic acid, 5 μM dopamine, 5 μM serotonin, 5 μM ADP and 5 μM AMP. The ATP biosensor was also shown to have excellent stability over 7 days, and showed only a 23.92 ± 3.55% loss in sensitivity. The ATP biosensor was utilised for the in vitro detection of ATP from gastrointestinal tissue. The ATP biosensor response was stable for 5h during in vitro recordings from ileum tissue. ATP release was shown to be greater from the mucosal surface in the ileum compared to the colon.
我们开发了一种稳定且选择性的 ATP 生物传感器，用于长期体外组织监测。该电极是通过将葡萄糖氧化酶（GOx）和己糖激酶（HEX）包埋在 Pt 微电极上的聚多酚膜中制成的。生物传感器对固定浓度的葡萄糖稳定超过 20 分钟，检测限为 9.9 ± 3.2 nM，灵敏度为 45.8 ± 1.22 pA μM(-1)。最重要的是，在存在 1mM 抗坏血酸、5 μM 多巴胺、5 μM 血清素、5 μM ADP 和 5 μM AMP 的情况下，ATP 生物传感器的响应没有改变。ATP 生物传感器在 7 天内也表现出出色的稳定性，仅损失 23.92 ± 3.55%的灵敏度。该 ATP 生物传感器还用于胃肠道组织中 ATP 的体外检测。在从回肠组织进行的体外记录期间，ATP 生物传感器的响应在 5 小时内保持稳定。与结肠相比，ATP 释放从回肠的黏膜表面更大。
