Dipartimento di Scienza dei Materiali, Università del Salento, via Arnesano, 73100 Lecce, Italy.
Analyst. 2011 Jan 7;136(1):164-73. doi: 10.1039/c0an00194e. Epub 2010 Sep 29.
A simple and novel amperometric biosensor for glucose detection is proposed. It is based on the immobilization of glucose oxidase (GOx) in a poly(vinyl alcohol) (PVA) matrix directly drop casted on a platinum electrode surface (Pt/GOx-PVA). Glucose was determined in the absence of a mediator used to transfer electrons between the electrode and the enzyme. The correlation between peak current (i(p)) and scan rate has been verified and the effect of pH solution has been checked. Glucose detection has been performed amperometrically at -400 mV by using pulsed amperometric detection (PAD). Under the selected optimal conditions, the biosensor showed low detection limit (10 μM), wide dynamic range (0.1-37 mM) and high sensitivity. The biosensor amperometric response revealed it to be specific to glucose without significant interference from other sugars and electroactive species coexisting with glucose in biological fluids. Response stability was another interesting feature of the developed system as it was almost completely recovered when the biosensor was left in opportune storage conditions (i.e., a response decrease of only 13% after 35 days in air at room temperature). Finally, X-Ray Photoelectron Spectroscopy (XPS) characterization revealed a homogeneous film deposited on the Pt substrate whose structure is also preserved under operative conditions.
提出了一种用于葡萄糖检测的简单新颖的安培生物传感器。它基于将葡萄糖氧化酶（GOx）直接固定在聚（乙烯醇）（PVA）基质中，然后直接滴铸在铂电极表面（Pt/GOx-PVA）上。在没有用于在电极和酶之间传递电子的介质的情况下测定葡萄糖。已经验证了峰电流（i（p））与扫描速率之间的相关性，并检查了 pH 溶液的影响。通过使用脉冲安培检测（PAD），在-400 mV 下进行了葡萄糖的安培检测。在选择的最佳条件下，该生物传感器具有低检测限（10 μM），宽动态范围（0.1-37 mM）和高灵敏度。生物传感器的安培响应表明它对葡萄糖具有特异性，而不会对生物体液中与葡萄糖共存的其他糖和电化学物质产生显著干扰。作为开发系统的另一个有趣特征是，当将生物传感器存放在适当的存储条件下时，其响应几乎完全恢复（即在室温下空气放置 35 天后，响应仅下降 13％）。最后，X 射线光电子能谱（XPS）表征显示在 Pt 基底上沉积了均匀的薄膜，其结构在工作条件下也得以保留。
