Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, 130062, People's Republic of China.
Clin Immunol. 2011 Feb;138(2):231-8. doi: 10.1016/j.clim.2010.11.008. Epub 2010 Dec 15.
Florfenicol has been shown to possess anti-inflammatory activity. However, its possible use for asthma has not yet been studied. First we investigated the anti-inflammatory properties of florfenicol using mice asthma model. BALB/c mice were immunized and challenged by ovalbumin. Treatment with florfenicol caused a marked reduction in inflammatory cells and three Th2 type cytokines in the bronchoalveolar lavage fluids of mice. The levels of ovalbumin-specific IgE and airway hyperresponsiveness were significantly altered after treatment with florfenicol. Histological studies using H&E and AB-PAS staining demonstrate that florfenicol substantially inhibited ovalbumin-induced inflammatory cells infiltration in lung tissue and goblet cell hyperplasia in the airway. These results were similar to those obtained with dexamethasone treatment. We then investigated which signal transduction mechanisms could be implicated in florfenicol activity. Our results suggested that the protective effect of florfenicol was mediated by the inhibition of the p38 MAPK-mediated phosphorylation of GATA 3.
氟苯尼考具有抗炎活性。然而，其在哮喘中的应用尚未得到研究。首先，我们使用小鼠哮喘模型研究了氟苯尼考的抗炎特性。BALB/c 小鼠用卵清蛋白免疫和激发。氟苯尼考治疗可显著减少小鼠支气管肺泡灌洗液中的炎症细胞和三种 Th2 型细胞因子。氟苯尼考治疗后，卵清蛋白特异性 IgE 和气道高反应性显著改变。H&E 和 AB-PAS 染色的组织学研究表明，氟苯尼考可显著抑制卵清蛋白诱导的肺组织炎症细胞浸润和气道杯状细胞增生。这些结果与地塞米松治疗的结果相似。然后，我们研究了哪些信号转导机制可能与氟苯尼考的活性有关。我们的结果表明，氟苯尼考的保护作用是通过抑制 p38 MAPK 介导的 GATA 3 磷酸化来介导的。
