Simoniello María Fernanda, Kleinsorge Elisa C, Carballo Marta A
Cátedra de Toxicología, Farmacología y Bioquímica Legal, FBCB, UNL, Ciudad Universitaria, Santa Fe, Argentina.
Medicina (B Aires). 2010;70(6):489-98.
Pesticides are used in agriculture to protect crops but may represent a potential risk to farmers and the environment. The aim of this work was to evaluate horticultural workers exposed to pesticide, categorized by: direct exposure (n = 45), indirect exposure (n = 50) and controls (n = 50) using exposure and effect biomarkers: cholinesterase (ChE), acetylcholinesterase (AChE), catalase (CAT), lipid peroxidation (TBARS), Damage Index Comet Assay (IDEC) and Damage Index Repair Assay (IDER). Our results show: a) an AChE inhibition in directly and indirectly exposed population (p < 0.001), b) significant increase in the levels of TBARS in direct exposure (p < 0.001), c) the CAT reduction was significant (p < 0.01), d) a significant increase in IDEC and IDER in both exposed groups (p < 0.001). Our results evidence variations in oxidative balance and DNA damage in exposed workers. These findings represent a contribution to the sub-clinical evaluation of subjects exposed to agrochemicals in our country.
农药在农业中用于保护作物，但可能对农民和环境构成潜在风险。这项工作的目的是使用暴露和效应生物标志物：胆碱酯酶（ChE）、乙酰胆碱酯酶（AChE）、过氧化氢酶（CAT）、脂质过氧化（TBARS）、彗星试验损伤指数（IDEC）和损伤修复指数（IDER），对接触农药的园艺工人进行评估，分为：直接暴露组（n = 45）、间接暴露组（n = 50）和对照组（n = 50）。我们的结果显示：a）直接和间接暴露人群中AChE受到抑制（p < 0.001），b）直接暴露组中TBARS水平显著升高（p < 0.001），c）CAT显著降低（p < 0.01），d）两个暴露组的IDEC和IDER均显著增加（p < 0.001）。我们的结果证明了暴露工人氧化平衡和DNA损伤的变化。这些发现为我国接触农用化学品的受试者的亚临床评估做出了贡献。
