Graduate student, Department of Dental Prosthodontics, Seoul National University College of Dentistry, Seoul, Korea.
J Adv Prosthodont. 2009 Jul;1(2):91-6. doi: 10.4047/jap.2009.1.2.91. Epub 2009 Jul 31.
The aim of this study was to study the effects of various surface treatments to a titanium surface on the expression of Runx2 in vitro.
Human Osteosarcoma TE-85 cells were cultured on machined, sandblasted, or anodic oxidized cpTi discs. At various times of incubation, the cells were collected and then processed for the analysis of mRNA expression of Runx2 using reverse transcription-PCR.
The expression pattern of Runx2 mRNA was differed according to the types of surface treatment. When the cells were cultured on the untreated control culture plates, the gene expression of Runx2 was not increased during the experiments. In the case of that the cells were cultured on the machined cpTI discs, the expression level was intermediate at the first day, but increased constitutively to day 5. In cells on sandblasted cpTi discs, the expression level was highest in the first day sample and the level was maintained to 5 days. In cells on anodized cpTi discs, the expression level increased rapidly to 3 days, but decreased slightly in the 5-th day sample.
Different surface treatments may contribute to the regulation of osteoblast function by influencing the level of gene expression of key osteogenic factors.
本研究旨在研究钛表面的各种表面处理对体外 Runx2 表达的影响。
将人骨肉瘤 TE-85 细胞培养在机加工、喷砂或阳极氧化 cpTi 盘上。在不同的孵育时间，收集细胞，然后使用逆转录-PCR 分析 Runx2 的 mRNA 表达。
Runx2 mRNA 的表达模式根据表面处理的类型而不同。当细胞在未处理的对照培养板上培养时，实验过程中 Runx2 的基因表达没有增加。当细胞在机加工 cpTI 盘上培养时，第 1 天的表达水平中等，但持续增加至第 5 天。在喷砂 cpTi 盘上的细胞中，第 1 天的表达水平最高，第 5 天的表达水平保持不变。在阳极氧化 cpTi 盘上的细胞中，表达水平迅速增加至第 3 天，但在第 5 天的样本中略有下降。
不同的表面处理方法可能通过影响关键成骨因子的基因表达水平来调节成骨细胞的功能。
