Schneider G B, Perinpanayagam H, Clegg M, Zaharias R, Seabold D, Keller J, Stanford C
N402, Dows Institute for Dental Research and the Department of Prosthodontics, University of Iowa College of Dentistry, Iowa City 52242, USA.
J Dent Res. 2003 May;82(5):372-6. doi: 10.1177/154405910308200509.
The transcription factor Cbfa1 regulates osteoblast differentiation and expression of genes necessary for the development of a mineralized phenotype. The purpose of this study was to determine if Cbfa1 and BSPII gene expression are influenced by implant surface microtopography. Osteoblasts were cultured on 600-grit (grooved) or sandblasted (roughened) cpTi implant discs. Mineralization was evaluated by Alizarin-Red-S staining. Real Time PCR was used for quantitative analysis of Cbfa1 and BSPII gene expression. Enhanced mineralization was seen in osteoblasts grown on roughened implant surfaces relative to tissue culture plastic. Real Time PCR showed significant (P < 0.05) increases in Cbfa1 gene expression in cells grown on roughened, as compared with grooved, implant surfaces. BSPII gene expression was also increased on rough surfaces in the UMR cells, but was reduced in the rat calvarial osteoblast cultures. These results suggest that osteoblast gene expression and mineralization are affected by roughened implant surface microtopographies during osseointegration of dental implants.
转录因子Cbfa1调节成骨细胞分化以及矿化表型发育所需基因的表达。本研究的目的是确定Cbfa1和骨唾液蛋白II（BSPII）基因表达是否受种植体表面微观形貌的影响。将成骨细胞培养在600目（带凹槽）或喷砂（粗糙化）的纯钛种植体圆盘上。通过茜素红S染色评估矿化情况。采用实时定量聚合酶链反应（Real Time PCR）对Cbfa1和BSPII基因表达进行定量分析。与组织培养塑料相比，在粗糙种植体表面生长的成骨细胞矿化增强。实时定量聚合酶链反应显示，与带凹槽的种植体表面相比，在粗糙种植体表面生长的细胞中Cbfa1基因表达显著增加（P < 0.05）。UMR细胞在粗糙表面上BSPII基因表达也增加，但在大鼠颅骨成骨细胞培养物中则降低。这些结果表明，在牙种植体骨结合过程中，粗糙的种植体表面微观形貌会影响成骨细胞基因表达和矿化。
