Department of Agriculture and Ecology, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark.
Curr Microbiol. 2011 Apr;62(4):1185-92. doi: 10.1007/s00284-010-9846-4. Epub 2010 Dec 17.
A rhizobacterium with high antifungal activity was isolated from a potato field at Inneruulalik, South Greenland. Phylogenetic analysis based on multi locus sequence typing showed that the bacterium was affiliated with strains of Pseudomonas fluorescens. The bacterium, denoted as Pseudomonas fluorescens In5, inhibited in vitro a broad range of phytopathogenic fungi, and the antifungal activity increased with decreasing temperature. Microcosm experiments demonstrated that P. fluorescens In5 protected tomato seedlings from Rhizoctonia solani. Transposon mutagenesis showed that the major cause for the antifungal activity of P. fluorescens In5 was a novel non-ribosomal peptide synthase (NRPS) gene. In addition, transposon mutagenesis showed that P. fluorescens In5 also contained a putative quinoprotein glucose dehydrogenase gene, which was involved in growth inhibition of phytopathogenic fungi. Although P. fluorescens In5 contained the capacity to synthesize hydrogen cyanide, β-1,3-glucanase, protease, and chitinase, these did not seem to play a role in the in vitro and microcosm antifungal assays.
从格陵兰南部因纽利拉克的一个马铃薯田中分离到一株具有高抗真菌活性的根际细菌。基于多位点序列分型的系统发育分析表明，该细菌与荧光假单胞菌的菌株有关。该细菌被命名为荧光假单胞菌 In5，它在体外抑制了广泛的植物病原真菌，并且抗真菌活性随着温度的降低而增加。微宇宙实验表明，荧光假单胞菌 In5 可保护番茄幼苗免受立枯丝核菌的侵害。转座子诱变表明，荧光假单胞菌 In5 产生抗真菌活性的主要原因是一种新型非核糖体肽合成酶（NRPS）基因。此外，转座子诱变表明，荧光假单胞菌 In5 还含有一个假定的醌蛋白葡萄糖脱氢酶基因，该基因参与了植物病原真菌的生长抑制。尽管荧光假单胞菌 In5 具有合成氢氰酸、β-1,3-葡聚糖酶、蛋白酶和几丁质酶的能力，但这些似乎在体外和微宇宙抗真菌测定中不起作用。
