假单胞菌属中氰化氢生物合成基因座hcnAB的聚合酶链反应扩增
PCR amplification of hydrogen cyanide biosynthetic locus hcnAB in Pseudomonas spp.
作者信息
Svercel M, Duffy B, Défago G
机构信息
Plant pathology, Institute of Integrative Biology, ETH-Zürich, CH-8092 Zürich, Switzerland.
出版信息
J Microbiol Methods. 2007 Jul;70(1):209-13. doi: 10.1016/j.mimet.2007.03.018. Epub 2007 Apr 6.
A PCR-based assay targeting hcnAB, essential genes for hydrogen cyanide (HCN) biosynthesis, allowed sensitive detection of HCN(+) pseudomonads between logs 2.9 and 3.5 cells per PCR reaction tube. RFLP analysis revealed 13 allele combinations among selected 2,4-diacetylphloroglucinol-producing (Phl(+))HCN(+), and 13 alleles in Phl(-) HCN(+) strains from a global collection.
一种基于聚合酶链反应（PCR）的检测方法靶向氰化氢（HCN）生物合成的必需基因hcnAB，能够灵敏地检测出每个PCR反应管中2.9至3.5个细胞之间的HCN(+)假单胞菌。限制性片段长度多态性（RFLP）分析揭示了从全球样本中选取的产2,4-二乙酰间苯三酚（Phl(+)）的HCN(+)菌株中有13种等位基因组合，以及Phl(-)的HCN(+)菌株中有13个等位基因。
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