Zhou Lin, Sun Hao, Xu Jia, Kang Jianfang
Huaian Hospital of Xuzhou Medical College,Huaian 223002,China.
Yan Ke Xue Bao. 2010 Aug;25(1):26-30. doi: 10.3969/g.issn.1000-4432.2010.01.007.
To explore the role of vascular endothelial growth factor and interleukin-6 in the aqueous humor in the pathogenesis of diabetic retinopathy(DR).
Aqueous humor samples were collected in type 2 diabetic patients during cataract surgery. The classification of DR was assigned based on the fundus examination and fluorescein angiography after cataract surgery. The experiment group included non-diabetic retinopathy (NDR,21 cases),background diabetic retinopathy (BDR,26 cases) and Proliferative diabetic retinopathy (PDR,19 cases). The control group was the healthy cataract patients (20 cases). The levels of VEGF and IL-6 in the aqueous humor was assessed using the ELISA technique.
The level of VEGF in NDR,BDR and PDR cases were(240.30+/-26.15)pg/ml, (292.27+/-58.91)pg/ml and (477.41+/-91.01)pg/m1, respectively. The level of IL-6 in NDR, BDR and PDR cases were (160.83+/-33.41)pg/ml, (238.60+/-62.23)pg/ml and (389.13+/-90.35)pg/ml,respectively. The levels of VEGF and IL-6 in the aqueous humour from the control group were (140.58+/-26.27) pg/ml,(82.72+/-21.53)pg/ml.The level of VEGF and IL-6 were significantly different in experiment and control groups(F=113.67,p<0.01; F=106.53, p<0.01). In the aqueous humor of diabetic patients increased significantly(p<0.O1). The level of VEGF and IL-6 was significantly correlated with each other in experiment group (r=0.995,p<0.O1). The levels of VEGF(r=0.792,0.826,0.841,p<0.01)and IL-6(r=0.829, 0.817.0.896,p<0.01)were also significantly correlated with the duration of DR.
VEGF and IL-6 play important roles in the development of DR. The levels of these two factors are correlated with each other in DR patients.
探讨房水中血管内皮生长因子和白细胞介素-6在糖尿病视网膜病变（DR）发病机制中的作用。
收集2型糖尿病患者白内障手术期间的房水样本。根据白内障手术后的眼底检查和荧光素血管造影对DR进行分类。实验组包括非糖尿病性视网膜病变（NDR，21例）、背景性糖尿病视网膜病变（BDR，26例）和增殖性糖尿病视网膜病变（PDR，19例）。对照组为健康白内障患者（20例）。采用酶联免疫吸附测定（ELISA）技术评估房水中VEGF和IL-6的水平。
NDR、BDR和PDR病例中VEGF水平分别为（240.30±26.15）pg/ml、（292.27±58.91）pg/ml和（477.41±91.01）pg/ml。NDR、BDR和PDR病例中IL-6水平分别为（160.83±33.41）pg/ml、（238.60±62.23）pg/ml和（389.13±90.35）pg/ml。对照组房水中VEGF和IL-6水平分别为（140.58±26.27）pg/ml、（82.72±21.53）pg/ml。实验组和对照组中VEGF和IL-6水平差异有统计学意义（F=113.67，p<0.01；F=106.53，p<0.01）。糖尿病患者房水中VEGF和IL-6水平显著升高（p<0.01）。实验组中VEGF和IL-6水平显著相关（r=0.995，p<0.01）。VEGF（r=0.792、0.826、0.841，p<0.01）和IL-6（r=0.829、0.817、0.896，p<0.01）水平也与DR病程显著相关。
VEGF和IL-6在DR发展中起重要作用。这两种因子水平在DR患者中相互关联。
