Shi Li-juan, Tang Hao
Hearing Research Laboratory, Basic Medical Department, China Medical University, Shenyang 110001, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2005 Feb;21(1):96-9.
To investigate the expression of Inducible Nitric Oxide Synthase (iNOS) in the cochlea of guinea pig after streptomycin (SM) and the antagonism of Salvia Miltiorrhiza injection on SM ototoxicity.
Light microscope, transmission electron microscope (TEM), immunohistochemical staining and image quantitative analysis technique, combined with auditory brainstem response (ABR) measurement were used.
After 10 days by drugs, the threshold of ABR of SM increased significantly, and the threshold of ABR of DS+ SM reduced more than that of SM (P < 0.01), the Corti's organ (CO), inner hair cell (IHC) and outer hair cell (OHC), spiral ganglion(SG), stria vascularis (SV) of SM damaged more greatly than that of DS + SM under light microscope and TEM. The result of immunohistochemical staining implied the expression of iNOS in CO, IHC and OHC, SG, SV of SM higher than that of DS+ SM.
The threshold of ABR increases and the expression of iNOS strengthens on SM ototoxicity. DS can reduce the increasing and inhibit the over-expression of iNOS, and reduce the damage of SM ototoxicity, which implies the protective role of DS on SM ototoxicity.
探讨链霉素（SM）致豚鼠耳蜗中诱导型一氧化氮合酶（iNOS）的表达及丹参注射液对SM耳毒性的拮抗作用。
采用光镜、透射电镜（TEM）、免疫组化染色及图像定量分析技术，并结合听性脑干反应（ABR）检测。
用药10天后，SM组ABR阈值显著升高，丹参+SM组ABR阈值降低幅度大于SM组（P<0.01）；光镜及TEM下，SM组柯蒂氏器（CO）、内毛细胞（IHC）、外毛细胞（OHC）、螺旋神经节（SG）、血管纹（SV）损伤程度大于丹参+SM组。免疫组化染色结果显示，SM组CO、IHC、OHC、SG、SV中iNOS的表达高于丹参+SM组。
SM耳毒性可致ABR阈值升高及iNOS表达增强。丹参可降低ABR阈值升高幅度，抑制iNOS的过度表达，减轻SM耳毒性损伤，提示丹参对SM耳毒性具有保护作用。
