Shi Li-juan, Guan Jie, Tang Hao
Department of Physiology, China Medical University, Shengyang 110001, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2009 May;25(2):264-7.
To study the expression of iNOS and AChE on ginea pigs cochlea spiral ganglion induced by streptomycin (SM) and attenuation by salvia miltiorrhiza injection (Chinese Traditional medicine-dansen DS).
32 guinea pigs were divided into 4 groups randomly (n=8): control group, SM group, DS + SM group, DS group. SABC immunohistochemical staining and image quantitative analysis technique were used to observe the expression of iNOS and AChE, as well as grey value analysis, and ABR measurements were used to observe ototoxicity.
After 10 days with drugs, the ABR threshold value of SM increased more significantly than that of the control (P < 0.01), while the ABR threshold value of DS+ SM co-treatment increased than the control group, but lower than that of SM group (P < 0.01). The results of immunohistochemical staining implied the expression of iNOS and AChE in SG of SM group were higher than that of control group, and had positive correlate.
The ABR threshold value increases and the expression of iNOS and AChE strengthen on SM ototoxicity, and has some correlation. DS can attenuate the ototoxicity induced by SM, and has protective function.
研究链霉素（SM）对豚鼠耳蜗螺旋神经节诱导型一氧化氮合酶（iNOS）和乙酰胆碱酯酶（AChE）的表达影响以及丹参注射液（中药-丹参DS）的减轻作用。
32只豚鼠随机分为4组（n = 8）：对照组、SM组、DS + SM组、DS组。采用SABC免疫组织化学染色和图像定量分析技术观察iNOS和AChE的表达以及灰度值分析，采用听性脑干反应（ABR）测量观察耳毒性。
用药10天后，SM组的ABR阈值升高比对照组更显著（P < 0.01），而DS + SM联合治疗组的ABR阈值高于对照组，但低于SM组（P < 0.01）。免疫组织化学染色结果表明，SM组螺旋神经节中iNOS和AChE的表达高于对照组，且呈正相关。
SM耳毒性导致ABR阈值升高，iNOS和AChE表达增强，且存在一定相关性。DS可减轻SM诱导的耳毒性，具有保护作用。
