Wang Ai-Mei, Tang Hao, Shen Jing, Cui Cheng
Department of Physiology, Jinzhou Medical College, Jinzhou 121001, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2004 Nov;20(4):406-9.
To investigate the effects of injectio Salvia Miltiorrhiza (SM) on gentamicin (GM)-induced free radical formation in guinea pig cochlea, and to explore possible mechanisms on GM-induced ototoxicity.
Biochemical assays of superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in guinea pig cochlea, combined with auditory brainstem response (ABR) measurement and transmission electron microscopic observation were used in this investigation.
SOD activity was significantly declined while MDA content was distinctly increased in cochlear tissues after GM injection (P < 0.01). Moreover, they were well correlated with auditory function damage (|r| > 0.7). Co-treatment with SM evidently enhanced SOD activity and decreased MDA content (P < 0.01, P < 0.05). Furthermore, auditory function was markedly ameliorated. Morphological changes of cochlea were consistent with those of hearing function.
Lipid peroxidation elicited by free radical was involved in GM-induced cochleotoxicity. SM might enhance SOD activity and prevent lipid peroxidation. As the result it might alleviate GM ototoxicity, and improve auditory function.
研究丹参注射液对庆大霉素诱导的豚鼠耳蜗自由基形成的影响，并探讨其对庆大霉素耳毒性的可能作用机制。
采用豚鼠耳蜗超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量的生化检测方法，并结合听性脑干反应（ABR）测量和透射电子显微镜观察进行本研究。
注射庆大霉素后，耳蜗组织中SOD活性显著下降，MDA含量明显增加（P<0.01）。此外，它们与听觉功能损害密切相关（|r|>0.7）。丹参注射液联合治疗明显增强了SOD活性，降低了MDA含量（P<0.01，P<0.05）。此外，听觉功能明显改善。耳蜗的形态学变化与听力功能变化一致。
自由基引发的脂质过氧化参与了庆大霉素诱导的耳蜗毒性。丹参注射液可能增强SOD活性，预防脂质过氧化。结果可能减轻庆大霉素的耳毒性，改善听觉功能。
