Department of Plastic Surgery, Hand Surgery, and Burns, Linköping University Hospital, Linköping University, Linköping, Sweden.
Microcirculation. 2011 Apr;18(3):198-204. doi: 10.1111/j.1549-8719.2010.00077.x.
The aim of this study was to evaluate the urea clearance technique for the measurement of drug-induced blood flow changes in human skin and compare it to two non-invasive techniques: polarization light spectroscopy and laser Doppler perfusion imaging.
Fifteen microdialysis catheters were placed intracutaneously on the volar aspect of the forearms of healthy human subjects and were perfused with nitroglycerine, noradrenaline, and again nitroglycerine to induce local tissue hyperemia, hypoperfusion, and hyperemia, respectively.
Urea clearance, but not the other techniques, detected the changes in blood flow during changes in flow. The last hyperemic response was detected by all three methods.
Urea clearance can be used as a relatively simple method to estimate blood flow changes during microdialysis of vasoactive substances, in particular when the tissue is preconditioned in order to enhance the contrast between baseline and the responses to the provocations. Our results support that, in the model described, urea clearance was superior to the optical methods as it detected both the increases and decrease in blood flow, and the returns to baseline between these periods.
本研究旨在评估尿素清除技术在测量人皮肤中药物诱导的血流变化中的应用，并将其与两种非侵入性技术：偏振光光谱法和激光多普勒灌注成像进行比较。
将 15 个微透析导管置于健康人体前臂的掌侧皮内，并分别用硝酸甘油、去甲肾上腺素和硝酸甘油再次灌注，以分别诱导局部组织充血、低灌注和再充血。
尿素清除技术（但不是其他技术）可以检测到血流变化期间的血流变化。所有三种方法都检测到了最后一次的充血反应。
尿素清除技术可作为一种相对简单的方法，用于估计血管活性物质微透析过程中的血流变化，特别是在组织预处理以增强基线和刺激反应之间的对比度的情况下。我们的结果支持在描述的模型中，尿素清除技术优于光学方法，因为它可以检测到血流的增加和减少，以及在这些期间之间返回基线。
