School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia.
J Ethnopharmacol. 2011 Mar 24;134(2):221-7. doi: 10.1016/j.jep.2010.12.007. Epub 2010 Dec 15.
Gynura segetum is a popular medicinal plant in Indonesia and Malaysia, known to possess various medicinal properties especially for treatment of cancer, diabetes and hypertension.
This study was carried out to evaluate the anti-angiogenic effect of Gynura segetum leaves extracts and its fractions. The chemical compositions of the active extracts were also determined.
The anti-angiogenic activity of Gynura segetum leaves extracts and its fractions was evaluated in vivo using the chick embryo chorioallantoic membrane (CAM) assay. Gas chromatography-mass spectrometry (GC-MS) analysis was carried out to identify the chemical compositions of the active extracts.
The CAM treated with Gynura segetum leaves extracts and its fractions (100μg/disc) showed a significantly greater anti-angiogenic effect compared to the positive control suramin (50μg/disc). Chemical analysis of the active extracts from the leaves of Gynura segetum yielded nine known compounds: undecane (1), neophytadine (2), hexadecanoic acid, methyl ester (3), 9,12-octadecadienoic acid, methyl ester (4), 9,12,15-octadecatrienoic acid, methyl ester (5), phytol (6), tetradecanal (7), octadecanoic acid, methyl ester (8) and γ-sitosterol (9).
These results suggested that Gynura segetum has anti-angiogenic activity. The plant may be used as a potential source for protection against cancer.
三叶鬼针草是印度尼西亚和马来西亚流行的药用植物，具有多种药用特性，尤其可用于治疗癌症、糖尿病和高血压。
本研究旨在评估三叶鬼针草叶提取物及其馏分的抗血管生成作用。还确定了活性提取物的化学成分。
使用鸡胚绒毛尿囊膜（CAM）试验在体内评估三叶鬼针草叶提取物及其馏分的抗血管生成活性。进行气相色谱-质谱（GC-MS）分析以鉴定活性提取物的化学成分。
用三叶鬼针草叶提取物及其馏分（100μg/disc）处理的 CAM 显示出比阳性对照苏拉明（50μg/disc）更强的抗血管生成作用。从三叶鬼针草叶的活性提取物的化学分析得到了九个已知化合物：十一烷（1）、新 Phytadine（2）、十六烷酸甲酯（3）、9,12-十八碳二烯酸甲酯（4）、9,12,15-十八碳三烯酸甲酯（5）、叶绿醇（6）、十四醛（7）、十八烷酸甲酯（8）和γ-谷甾醇（9）。
这些结果表明三叶鬼针草具有抗血管生成活性。该植物可作为预防癌症的潜在来源。
