School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Toxicol In Vitro. 2010 Sep;24(6):1655-61. doi: 10.1016/j.tiv.2010.05.022. Epub 2010 Jun 4.
This study has been undertaken to elucidate the anti-angiogenic properties of shallot extract in vitro and in vivo and also to define the responsible fraction and its stability. After preparation of the extract of shallot bulbs with 50% ethanol, the extract was successively fractionated into n-hexane, ethyl acetate, n-butanol and aqueous fractions. The ethyl acetate fraction was further fractionated to three subfractions using thin layer chromatography. Anti-angiogenic activity of fractions and subfractions were examined on human umbilical vein endothelial cells (HUVECs) in collagen matrix and chicken chorioallantoic membrane (CAM) models. Among the fractions, ethyl acetate fraction and one of its subfractions potently inhibited angiogenesis in vitro and in vivo. Furthermore, ethyl acetate fraction sustained its inhibitory effect significantly even after treatment in high thermal and low pH conditions. These findings provided a useful basis for further investigations on shallot as a useful herb with therapeutic or preventive activity against angiogenesis related disorders.
本研究旨在阐明洋葱提取物的体外和体内抗血管生成特性，并确定其负责的部分及其稳定性。洋葱鳞茎用 50%乙醇提取后，提取物依次分为正己烷、乙酸乙酯、正丁醇和水部分。乙酸乙酯部分进一步用薄层层析法分为三个亚部分。在胶原基质和鸡胚绒毛尿囊膜 (CAM) 模型中，对各部分和亚部分的抗血管生成活性进行了检测。在各部分中，乙酸乙酯部分及其一个亚部分在体外和体内均能强烈抑制血管生成。此外，即使在高温和低 pH 条件下处理后，乙酸乙酯部分仍能显著维持其抑制作用。这些发现为进一步研究洋葱作为一种具有治疗或预防与血管生成相关疾病的活性的有用草药提供了有用的基础。
