Siragusa G R, Johnson M G
Department of Food Science, University of Arkansas, Fayetteville 72703.
Appl Environ Microbiol. 1990 Jun;56(6):1897-904. doi: 10.1128/aem.56.6.1897-1904.1990.
Eight hundred fifty-nine murine hybridomas were produced from eight fusions, and 27 were characterized for secretion of antibodies reactive to Listeria monocytogenes. One monoclonal antibody (MAb), P5C9, reacted with all test strains of L. monocytogenes (31 of 31), L. innocua (3 of 3), and L. welshimeri (1 of 1) but not with any strains of the other four Listeria species or with any of 22 gram-positive or 11 gram-negative species of bacteria when tested in microtiter and dot blot enzyme immunoassays. Of the other 26 antibodies, 20 reacted with either L. monocytogenes Scott A or V7 and with some or all of the other six Listeria species but also cross-reacted with some or all of the non-Listeria bacteria tested. MAb P5C9 is of the immunoglobulin G1 murine subclass. In Western blot (immunoblot) analyses, this MAb reacted with a single antigen with a molecular weight of 18,500, and it is shared in common with all three reactive species, L. monocytogenes, L. innocua, and L. welshimeri. This antigen was extracted with detergent and appeared to be cell bound.
通过八次融合产生了859个鼠杂交瘤，其中27个被鉴定为可分泌对单核细胞增生李斯特菌有反应的抗体。一种单克隆抗体（MAb）P5C9与所有测试的单核细胞增生李斯特菌菌株（31株中的31株）、无害李斯特菌菌株（3株中的3株）和威尔希默李斯特菌菌株（1株中的1株）发生反应，但在微量滴定和斑点印迹酶免疫测定中，与其他四种李斯特菌属的任何菌株或22种革兰氏阳性菌及11种革兰氏阴性菌中的任何一种均无反应。在其他26种抗体中，有20种与单核细胞增生李斯特菌斯科特A株或V7株以及部分或全部其他六种李斯特菌属发生反应，但也与部分或全部测试的非李斯特菌发生交叉反应。单克隆抗体P5C9属于免疫球蛋白G1鼠亚类。在蛋白质印迹（免疫印迹）分析中，这种单克隆抗体与一种分子量为18500的单一抗原发生反应，并且单核细胞增生李斯特菌、无害李斯特菌和威尔希默李斯特菌这三种有反应的菌属都共有这种抗原。这种抗原可用去污剂提取，且似乎与细胞结合。
