[The centrosome reaction in tissue-culture cells to depolarization of the cell membranes].

Carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP), ouabain and calcium ionophore A23187 caused a centrosome restructuring expressed in mean number increase of satellites on the active (mother) centriole, in an increase of the mean slope of centrioles to the substrate surface and in the more frequent occurrence of primary cilia. In the presence of FCCP the effect appeared only in 10 min and retained for 2 h. The ouabain caused the separation of active and inactive centrioles in more than in a half of the cells. The data obtained permit to conclude that depolarization of the plasmatic membrane only is needed for the initiation of centrosome restructuring. The authors propose that this reaction of centrosome is a component of the cell overall response to nonspecific lesions.