Kumar M Ratan, Reddy A Gopala, Anjaneyulu Y, Reddy G Dilip
Department of Pharmacology and Toxicology, College of Veterinary Science, Rajendranagar, Hyderabad - 500030, India.
Toxicol Int. 2010 Jul;17(2):45-8. doi: 10.4103/0971-6580.72668.
Effect of lead was studied for its action on antioxidant defense in broilers. A total of 225 one-day-old male broiler chicks (Vencobb strain) were divided randomly into 15 groups consisting of 15 chicks in each group. Group 1 was maintained on basal diet, group 2 on polyherbal formulation (PHF; stressroak), group 3 on shilajit, group 4 on amla, and group 5 on vitamin E + selenium (Se). Group 6 was maintained on lead for 42 days (6 weeks) and group 7 on lead for 28 days and subsequently on basal diet without lead for the remaining two weeks. Groups 8, 9, 10, and 11 were given lead along with PHF, shilajit, amla, and vitamin E + Se, respectively throughout the experiment for 6 weeks. Groups 12, 13, 14, and 15 were given lead containing diet for the first four weeks (28 days) and subsequently treated with PHF, shilajit, amla, and vitamin E + Se, respectively for the remaining two weeks. Antioxidant status of the birds was analyzed by assaying blood samples for glutathione (GSH) peroxidase, GSH reductase, and catalase at the end of fourth and sixth weeks, whereas Thiobarbituric acid reacting substances (TBARS) and GSH concentrations were estimated in liver homogenate at the end of the sixth week. The antioxidant defense parameters were significantly altered in toxic control groups indicating the possible oxidative damage caused by lead, whereas the parameters were normal in control groups 1 to 5 and other groups that were given the drugs in test, indicating their good ameliorating activity in oxidative stress.
研究了铅对肉鸡抗氧化防御系统的作用。总共225只1日龄雄性肉鸡雏鸡（Vencobb品系）被随机分为15组，每组15只雏鸡。第1组饲喂基础日粮，第2组饲喂多草药配方（PHF；应激缓解剂），第3组饲喂希拉季特，第4组饲喂余甘子，第5组饲喂维生素E + 硒（Se）。第6组饲喂铅42天（6周），第7组饲喂铅28天，随后在剩余两周饲喂无铅基础日粮。在整个实验的6周内，第8、9、10和11组分别在饲喂铅的同时给予PHF、希拉季特、余甘子和维生素E + Se。第12、13、14和15组在前四周（28天）饲喂含铅日粮，随后在剩余两周分别用PHF、希拉季特、余甘子和维生素E + Se进行处理。在第4周和第6周结束时，通过检测血样中的谷胱甘肽（GSH）过氧化物酶、GSH还原酶和过氧化氢酶来分析鸡的抗氧化状态，而在第6周结束时，在肝脏匀浆中测定硫代巴比妥酸反应物质（TBARS）和GSH浓度。在毒性对照组中，抗氧化防御参数发生了显著变化，表明铅可能造成了氧化损伤，而在对照组1至5以及接受受试药物的其他组中，参数正常，表明它们在氧化应激中具有良好的改善活性。
