Department of Molecular and Cell Biology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
PLoS One. 2010 Dec 8;5(12):e15318. doi: 10.1371/journal.pone.0015318.
Human cytomegalovirus (HCMV) completes its final envelopment on intracellular membranes before it is released from the cell. The mechanisms underlying these processes are not understood. Here we studied the role of Rab27a, a regulator of lysosome-related organelle transport, in HCMV production. HCMV infection increased Rab27a expression, and recruitment of Rab27a to membranous strutures at the assembly site. Immuno-gold labelling demonstrated association of Rab27a with viral envelopes. CMV production was reduced after knock-down of Rab27a, and in Rab27a-deficient ashen melanocytes. This study shows a requirement for Rab27a in the CMV life cycle and suggests that CMV and LRO biogenesis share common molecular mechanisms.
人巨细胞病毒 (HCMV) 在从细胞中释放之前，在细胞内膜上完成其最后的包膜。这些过程的机制尚不清楚。在这里，我们研究了 Rab27a 在 HCMV 产生中的作用，Rab27a 是溶酶体相关细胞器运输的调节剂。HCMV 感染增加了 Rab27a 的表达，并募集到装配部位的膜结构上。免疫金标记显示 Rab27a 与病毒包膜相关。Rab27a 敲低后 CMV 产量减少，在 Rab27a 缺陷的 ashen 黑素细胞中 CMV 产量也减少。本研究表明 Rab27a 在 CMV 生命周期中的必要性，并表明 CMV 和 LRO 的生物发生具有共同的分子机制。
