Department of Chemistry, Tehran Payamenoor University, Tehran, Iran.
J Sep Sci. 2011 Jan;34(1):77-82. doi: 10.1002/jssc.201000526.
A simple and efficient method, ionic liquid-based dispersive liquid-liquid microextraction combined with high-performance liquid chromatography-ultraviolet detection (HPLC-UV), has been applied for the extraction and determination of some antioxidants (Irganox 1010, Irganox 1076 and Irgafos 168) in water samples. The microextraction efficiency factors were investigated and optimized: 1-hexyl-3-methylimidazolium hexafluorophosphate [C(6)MIM][PF(6)] (0.06 g) as extracting solvent, methanol (0.5 mL) as disperser solvent without salt addition. Under the selected conditions, enrichment factors up to 48-fold, limits of detection (LODs) of 5.0-10.0 ng/mL and dynamic linear ranges of 25-1500 ng/mL were obtained. A reasonable repeatability (RSD≤11.8%, n=5) with satisfactory linearity (r(2)≥0.9954) of the results illustrated a good performance of the presented method. The accuracy of the method was tested by the relative recovery experiments on spiked samples, with results ranging from 85 to 118%. Finally, the method was successfully applied for determination of the analytes in several real water samples.
一种简单高效的方法，离子液体分散液液微萃取结合高效液相色谱-紫外检测（HPLC-UV），已被应用于水样中一些抗氧化剂（Irganox 1010、Irganox 1076 和 Irgafos 168）的提取和测定。考察并优化了微萃取效率因素：1-己基-3-甲基咪唑六氟磷酸盐[C（6）MIM][PF（6）]（0.06 g）作为萃取溶剂，甲醇（0.5 mL）作为分散溶剂，不加盐。在选定的条件下，富集因子高达 48 倍，检测限（LOD）为 5.0-10.0 ng/mL，动态线性范围为 25-1500 ng/mL。该方法具有良好的重现性（RSD≤11.8%，n=5）和令人满意的线性（r（2）≥0.9954），表明该方法具有良好的性能。通过对加标样品的相对回收率实验对方法的准确性进行了验证，结果在 85%至 118%之间。最后，该方法成功应用于几种实际水样中分析物的测定。
