Cao Pingping, Hua Xiaoyang, Liu Zheng, Chu Hanqi, Cui Yonghua
Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2010 Oct;24(20):921-3.
To observe the pathologic characteristics, and investigate mast cell and its activation in chronic rhinosinusitis without nasal polyps (CRSsNP) and their relations with eosinophilic inflammation.
HE stain was used to observe tissue features and count total inflammatory cells, mononuclear cells, plasma cells and eosinophilic in lamina propria of CRSsNP and inferior turbinate. Toluidine blue stain and immunohistochemical stain for tryptase were used to detect mast cell and its activation respectively in CRSsNP and control, and their corelations with tissue eosinophilia were analysed.
CRSsNP has increased total inflammatory cells, mononuclear cells and plasma cells but comparable eosinophilic and lamina propria glands compared with inferior turbinate. Mast cells corelated with activated mast cells, but there was no difference between CRSsNP and control for both of them and there were no corelation between mast cell and its activation with tissue eosinophilia.
CRSsNP has more serious inflammation but no more mast cell and its activation and eosinophil compared with inferior turbinate, and there were no corelations between mast cell and its activation with eosinophil count which suggests that mast cell and eosinophilic inflammation mediated by it may not play an important role in the pathogenesis of CRSsNP.
观察非鼻息肉性慢性鼻窦炎（CRSsNP）的病理特征，研究肥大细胞及其活化情况，并探讨其与嗜酸性粒细胞炎症的关系。
采用苏木精-伊红（HE）染色观察CRSsNP及下鼻甲固有层的组织特征，计数总炎症细胞、单核细胞、浆细胞和嗜酸性粒细胞。分别用甲苯胺蓝染色和抗胰蛋白酶免疫组化染色检测CRSsNP组和对照组中的肥大细胞及其活化情况，并分析它们与组织嗜酸性粒细胞增多的相关性。
与下鼻甲相比，CRSsNP组的总炎症细胞、单核细胞和浆细胞增多，但嗜酸性粒细胞和固有层腺体数量相当。肥大细胞与活化肥大细胞相关，但CRSsNP组与对照组在这两者之间无差异，且肥大细胞及其活化与组织嗜酸性粒细胞增多之间无相关性。
与下鼻甲相比，CRSsNP炎症更严重，但肥大细胞及其活化以及嗜酸性粒细胞数量并无增多，且肥大细胞及其活化与嗜酸性粒细胞计数之间无相关性，这表明肥大细胞及其介导的嗜酸性粒细胞炎症可能在CRSsNP发病机制中不起重要作用。
