Chen Jia-Xu, Tang Yi-Ting
Beijing University of Traditional Chinese Medicine, Beijing 100029, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2004 Feb;20(1):71-4.
To observe the effect of Xiaoyao Powder on the content changes of CRF mRNA in relative brain zone (hypothalamus, pituitary, hippocampus, cortex) in chronic restrained stress rats.
RT-PCR and graphic analysis methods were applied to test the content changes of CRF mRNA in relative brain zone.
The CRF-1 gene expression in hypothalamus was modulated lower in stress group and the difference was significant when compared with control group (P < 0.01). The gene expression of CRF-1 in Xiaoyao Powder group was markedly modulated lower in hypothalamus, but it was markedly modulated higher in cortex as compared with control group (P < 0.01). The gene expression of CRF-2 in Xiaoyao Powder group was higher in hypothalamus than that in stress group (P < 0.01), and it was also higher in hippocampus as compared with that in control and stress group (P < 0.01, P < 0.05 respectively).
The modulation point of Xiaoyao Powder group on central neuropeptide of chronic restrained stress is respectively hypothalamus, pituitary, hippocampus and cortex. The modulating target is hypothalamus, the limbic system and the cortex center. It can be suggested that medicine of smoothing the liver simultaneously have poly-target and dual-modulating action and been involved in the integration function of nerve-endocrine-immune net.
观察逍遥散对慢性束缚应激大鼠相对脑区（下丘脑、垂体、海马、皮层）促肾上腺皮质激素释放因子（CRF）mRNA含量变化的影响。
采用逆转录-聚合酶链反应（RT-PCR）及图像分析方法检测相对脑区CRF mRNA的含量变化。
应激组下丘脑CRF-1基因表达下调，与对照组比较差异有统计学意义（P<0.01）。逍遥散组下丘脑CRF-1基因表达明显下调，但与对照组比较，皮层CRF-1基因表达明显上调（P<0.01）。逍遥散组下丘脑CRF-2基因表达高于应激组（P<0.01），与对照组和应激组比较，海马CRF-2基因表达也升高（分别为P<0.01，P<0.05）。
逍遥散组对慢性束缚应激大鼠中枢神经肽的调节点分别在下丘脑、垂体、海马和皮层，调节靶点为下丘脑、边缘系统和皮层中枢。提示疏肝类中药具有多靶点、双向调节作用，参与神经-内分泌-免疫网络的整合功能。
