Kvist Sebastian, Oceguera-Figueroa Alejandro, Siddall Mark E, Erséus Christer
Division of Invertebrate Zoology, Richard Gilder Graduate School, American Museum of Natural History, New York 10024, USA.
Mitochondrial DNA. 2010 Dec;21(6):198-205. doi: 10.3109/19401736.2010.529905.
Species identifications based on DNA barcoding rely on the correct identity of previously barcoded specimens, but little attention has been given to whether deposited barcodes include correspondence to the species' name-bearing type. The information content associated with COX1 sequences in the two most commonly used repositories of barcodes, GenBank and the Barcode of Life Data System (BOLD), is often insufficient for subsequent evaluation of the robustness of the identification procedure. We argue that DNA barcoding and taxonomy alike will benefit from more information content in the annotations of barcoded specimens as this will allow for validation and re-evaluation of the initial specimen identification. The aim should be to closely connect specimens from which reference barcodes are generated with the holotype through straight-forward taxonomy, and geographical and genetic correlations. Annotated information should also include voucher specimens and collector/identifier information. We examine two case studies based on empirical data, in which barcoding and taxonomy benefit from increased information content. On the basis of data from the first case study, we designate a barcoded neotype of the European medicinal leech, Hirudo medicinalis, on morphological and geographical grounds.
基于DNA条形码的物种鉴定依赖于先前已条形码化标本的正确身份，但对于所保存的条形码是否与物种的具名模式相对应却很少有人关注。在两个最常用的条形码数据库GenBank和生命条形码数据系统（BOLD）中，与COX1序列相关的信息内容往往不足以对鉴定程序的稳健性进行后续评估。我们认为，DNA条形码和分类学都将受益于条形码标本注释中更多的信息内容，因为这将允许对初始标本鉴定进行验证和重新评估。目标应该是通过直接的分类学、地理和遗传相关性，将产生参考条形码的标本与模式标本紧密联系起来。注释信息还应包括凭证标本以及采集者/鉴定者信息。我们基于实证数据研究了两个案例，其中条形码和分类学都受益于增加的信息内容。根据第一个案例研究的数据，我们基于形态学和地理学依据指定了欧洲医用水蛭Hirudo medicinalis的一个条形码新模标本。
