Department of Immunology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
Biochem Biophys Res Commun. 2011 Jan 21;404(3):822-8. doi: 10.1016/j.bbrc.2010.12.067. Epub 2010 Dec 21.
The intestinal epithelium contains columnar epithelial cells (ECs) and M cells, and fucosylation of the apical surface of ECs and M cells is involved in distinguishing the two populations and in their response to commensal flora and environmental stress. Here, we show that fucosylated ECs (F-ECs) were induced in the mouse small intestine by the pro-inflammatory agents dextran sodium sulfate and indomethacin, in addition to an enteropathogen derived cholera toxin. Although F-ECs showed specificity for the M cell-markers, lectin Ulex europaeus agglutinin-1 and our monoclonal antibody NKM 16-2-4, these cells also retained EC-phenotypes including an affinity for the EC-marker lectin wheat germ agglutinin. Interestingly, fucosylation of Peyer's patch M cells and F-ECs was distinctly regulated by α(1,2)fucosyltransferase Fut1 and Fut2, respectively. These results indicate that Fut2-mediated F-ECs share M cell-related fucosylated molecules but maintain distinctive EC characteristics, Fut1 is, therefore, a reliable marker for M cells.
肠上皮含有柱状上皮细胞（ECs）和 M 细胞，ECs 和 M 细胞顶膜的岩藻糖基化参与了这两种细胞群的区分，以及它们对共生菌群和环境应激的反应。在这里，我们发现除了一种来源于病原体的霍乱毒素之外，促炎剂葡聚糖硫酸钠和吲哚美辛也可诱导小鼠小肠中出现岩藻糖基化的 ECs（F-ECs）。虽然 F-ECs 对 M 细胞标志物——凝集素 Ulex europaeus agglutinin-1 和我们的单克隆抗体 NKM 16-2-4 具有特异性，但这些细胞仍然保留了 EC 表型，包括对 EC 标志物凝集素麦胚凝集素的亲和力。有趣的是，Peyer 斑 M 细胞和 F-ECs 的岩藻糖基化分别由 α(1,2)岩藻糖基转移酶 Fut1 和 Fut2 明显调控。这些结果表明，Fut2 介导的 F-ECs 共享与 M 细胞相关的岩藻糖基化分子，但保留了独特的 EC 特征，因此 Fut1 是 M 细胞的可靠标志物。
