Giannasca P J, Giannasca K T, Falk P, Gordon J I, Neutra M R
Gastrointestinal Cell Biology Laboratory, Children's Hospital, Boston, Massachusetts.
Am J Physiol. 1994 Dec;267(6 Pt 1):G1108-21. doi: 10.1152/ajpgi.1994.267.6.G1108.
We have used a panel of lectins and antibodies to describe the composition of complex carbohydrates associated with M cells in various regions of the intestinal tract of adult BALB/c mice. The fucose-specific lectin Ulex europaeus agglutinin type I (UEA I) is a marker of M cells in the small intestine and recognized M cells at an early stage of differentiation. Subpopulations of M cells in a single follicle-associated epithelium (FAE) could be distinguished by different fucose-specific probes. Certain lectins revealed that M cells have basal processes that extend into the underlying lymphoid tissue. Colonic and rectal M cells display glycosylation patterns distinct from M cells of Peyer's patches and are characterized by terminal galactose. UEA I selectively adhered to Peyer's patch M cells in mucosal explants and in ligated intestinal loops in vivo. The lectin was taken up into endocytic vesicles and transported to the intra-epithelial pocket and other domains of the basolateral membrane. Thus M cell-specific glycoconjugates could serve as "receptors" for targeting of lectin-antigen conjugates to the mucosal immune system.
我们使用了一组凝集素和抗体来描述成年BALB/c小鼠肠道不同区域与M细胞相关的复合碳水化合物的组成。岩藻糖特异性凝集素欧洲荆豆凝集素I型（UEA I）是小肠中M细胞的标志物，并且在分化早期就能识别M细胞。单个滤泡相关上皮（FAE）中的M细胞亚群可以通过不同的岩藻糖特异性探针来区分。某些凝集素显示，M细胞具有延伸至下方淋巴组织的基底突起。结肠和直肠的M细胞表现出与派尔集合淋巴结M细胞不同的糖基化模式，其特征为末端半乳糖。UEA I在体内的黏膜外植体和结扎肠袢中选择性地黏附于派尔集合淋巴结的M细胞。该凝集素被摄取到内吞小泡中，并转运至上皮内陷窝和基底外侧膜的其他区域。因此，M细胞特异性糖缀合物可作为“受体”，用于将凝集素-抗原缀合物靶向黏膜免疫系统。
