Department of Laboratory Medicine, The University of Texas M.D. Anderson Cancer Center, Houston, 77054, USA.
Am J Clin Pathol. 2011 Jan;135(1):90-5. doi: 10.1309/AJCP98JNYHGCGMHK.
We reviewed HLA antibody testing results using an enzyme-linked immunosorbent assay (ELISA) for all male blood donors at our institution during a 3.5-month period to look for HLA immunization. Confirmatory testing of 33 blood samples positive for HLA class I and/or II antibodies was performed using the fluorescent bead method. A retrospective review of recipients of packed RBCs and platelets processed from these 33 HLA-immunized male donors were conducted to identify transfusion-related acute lung injury and cognate antigens. The agreement rates between the methods for HLA class I and II antibodies were 21% (7/33) and 6% (2/33), respectively. We noted HLA antibodies in the male donors corresponding to cognate antigens in 2 recipients of packed RBCs and in 3 recipients of platelets. Of 8 donors positive for HLA antibodies, 5 did not have a history of blood transfusion. We conclude that ELISA was too sensitive and had a high false-positive rate for the detection of HLA class II antibodies.
我们回顾了在我们机构的 3.5 个月期间对所有男性献血者进行的 HLA 抗体检测结果，以寻找 HLA 免疫。对 33 份 HLA 类 I 和/或 II 抗体阳性的血液样本进行了荧光珠法的确认性检测。对来自这 33 名 HLA 免疫男性供体的浓缩红细胞和血小板进行了回顾性分析，以确定输血相关的急性肺损伤和同源抗原。HLA 类 I 和 II 抗体检测方法的一致性率分别为 21%（7/33）和 6%（2/33）。我们在 2 名浓缩红细胞接受者和 3 名血小板接受者中发现了与男性供体 HLA 抗体相对应的同源抗原。在 8 名 HLA 抗体阳性的供体中，有 5 名没有输血史。我们得出结论，ELISA 对 HLA 类 II 抗体的检测过于敏感，假阳性率较高。
