Reducing background fluorescence reveals adhesions in 3D matrices.
作者信息
Kubow Kristopher E, Horwitz Alan Rick
出版信息
Nat Cell Biol. 2011 Jan;13(1):3-5; author reply 5-7. doi: 10.1038/ncb0111-3.
Abstract
摘要
相似文献
1
Reducing background fluorescence reveals adhesions in 3D matrices.
Nat Cell Biol. 2011 Jan;13(1):3-5; author reply 5-7. doi: 10.1038/ncb0111-3.
2
FAK and paxillin dynamics at focal adhesions in the protrusions of migrating cells.
Sci Rep. 2014 Aug 12;4:6024. doi: 10.1038/srep06024.
3
Leupaxin is similar to paxillin in focal adhesion targeting and tyrosine phosphorylation but has distinct roles in cell adhesion and spreading.
Cell Adh Migr. 2010 Oct-Dec;4(4):527-40. doi: 10.4161/cam.4.4.12399.
4
Paxillin and Hic-5 interaction with vinculin is differentially regulated by Rac1 and RhoA.
PLoS One. 2012;7(5):e37990. doi: 10.1371/journal.pone.0037990. Epub 2012 May 22.
5
Anti-correlation of HER2 and focal adhesion complexes in the plasma membrane.
PLoS One. 2020 Jun 8;15(6):e0234430. doi: 10.1371/journal.pone.0234430. eCollection 2020.
6
Rho-kinase dependent organization of stress fibers and focal adhesions in cultured fibroblasts.
Genes Cells. 2007 May;12(5):623-38. doi: 10.1111/j.1365-2443.2007.01073.x.
7
Investigating lasp-2 in cell adhesion: new binding partners and roles in motility.
Mol Biol Cell. 2013 Apr;24(7):995-1006. doi: 10.1091/mbc.E12-10-0723. Epub 2013 Feb 6.
8
Mechanical forces alter zyxin unbinding kinetics within focal adhesions of living cells.
J Cell Physiol. 2006 Apr;207(1):187-94. doi: 10.1002/jcp.20550.
9
Dynamics and distribution of paxillin, vinculin, zyxin and VASP depend on focal adhesion location and orientation.
Sci Rep. 2019 Jul 18;9(1):10460. doi: 10.1038/s41598-019-46905-2.
10
Molecular mechanism of vinculin activation and nanoscale spatial organization in focal adhesions.
Nat Cell Biol. 2015 Jul;17(7):880-92. doi: 10.1038/ncb3180. Epub 2015 Jun 8.
引用本文的文献
1
Direct Arp2/3-vinculin binding is required for pseudopod extension, but only on compliant substrates and in 3D.
iScience. 2025 May 9;28(6):112623. doi: 10.1016/j.isci.2025.112623. eCollection 2025 Jun 20.
2
Regulation of cell migration by extracellular matrix mechanics at a glance.
J Cell Sci. 2025 Apr 1;138(7). doi: 10.1242/jcs.263574. Epub 2025 Apr 4.
3
Combined assembloid modeling and 3D whole-organ mapping captures the microanatomy and function of the human fallopian tube.
Sci Adv. 2024 Sep 27;10(39):eadp6285. doi: 10.1126/sciadv.adp6285.
4
Curved adhesions mediate cell attachment to soft matrix fibres in 3D.
bioRxiv. 2023 Mar 19:2023.03.16.532975. doi: 10.1101/2023.03.16.532975.
5
Mitotic outcomes and errors in fibrous environments.
Proc Natl Acad Sci U S A. 2023 Mar 7;120(10):e2120536120. doi: 10.1073/pnas.2120536120. Epub 2023 Feb 27.
6
Lack of Paxillin phosphorylation promotes single-cell migration in vivo.
J Cell Biol. 2023 Mar 6;222(3). doi: 10.1083/jcb.202206078. Epub 2023 Feb 1.
7
Focal adhesion-mediated cell anchoring and migration: from in vitro to in vivo.
Development. 2022 May 15;149(10). doi: 10.1242/dev.200647. Epub 2022 May 19.
8
Peptide-PAINT Enables Investigation of Endogenous Talin with Molecular Scale Resolution in Cells and Tissues.
Chembiochem. 2021 Oct 1;22(19):2872-2879. doi: 10.1002/cbic.202100301. Epub 2021 Jul 30.
9
Ventral stress fibers induce plasma membrane deformation in human fibroblasts.
Mol Biol Cell. 2021 Aug 19;32(18):1707-1723. doi: 10.1091/mbc.E21-03-0096. Epub 2021 Jun 30.
10
A spatial model of YAP/TAZ signaling reveals how stiffness, dimensionality, and shape contribute to emergent outcomes.
Proc Natl Acad Sci U S A. 2021 May 18;118(20). doi: 10.1073/pnas.2021571118. Epub 2021 May 14.
本文引用的文献
1
Direct comparisons of the morphology, migration, cell adhesions, and actin cytoskeleton of fibroblasts in four different three-dimensional extracellular matrices.
Tissue Eng Part A. 2011 Mar;17(5-6):713-24. doi: 10.1089/ten.TEA.2010.0273. Epub 2010 Dec 7.
2
A distinctive role for focal adhesion proteins in three-dimensional cell motility.
Nat Cell Biol. 2010 Jun;12(6):598-604. doi: 10.1038/ncb2062. Epub 2010 May 16.
3
Plasticity of cell migration: a multiscale tuning model.
J Cell Biol. 2010 Jan 11;188(1):11-9. doi: 10.1083/jcb.200909003. Epub 2009 Dec 1.
4
One-dimensional topography underlies three-dimensional fibrillar cell migration.
J Cell Biol. 2009 Feb 23;184(4):481-90. doi: 10.1083/jcb.200810041. Epub 2009 Feb 16.
5
Environmental sensing through focal adhesions.
Nat Rev Mol Cell Biol. 2009 Jan;10(1):21-33. doi: 10.1038/nrm2593.
6
Actin and alpha-actinin orchestrate the assembly and maturation of nascent adhesions in a myosin II motor-independent manner.
Nat Cell Biol. 2008 Sep;10(9):1039-50. doi: 10.1038/ncb1763.
7
Improving the photostability of bright monomeric orange and red fluorescent proteins.
Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 2008 May 4.
8
Rapid leukocyte migration by integrin-independent flowing and squeezing.
Nature. 2008 May 1;453(7191):51-5. doi: 10.1038/nature06887.
9
Fibronectin fibrillogenesis regulates three-dimensional neovessel formation.
Genes Dev. 2008 May 1;22(9):1231-43. doi: 10.1101/gad.1643308.
10
A talin-dependent LFA-1 focal zone is formed by rapidly migrating T lymphocytes.
J Cell Biol. 2005 Jul 4;170(1):141-51. doi: 10.1083/jcb.200412032. Epub 2005 Jun 27.
Zotero 插件