Key Laboratory of Chem-Biosensing, Anhui Province, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China.
Langmuir. 2011 Feb 1;27(3):1224-31. doi: 10.1021/la1033433. Epub 2010 Dec 21.
An electrochemical immunosensor was studied for sensitive detection of Interleukin-6 (IL-6) based on a dual amplification mechanism resulting from Au nanoparticles (AuNP)-Poly-dopamine (PDOP) as the sensor platform and multienzyme-antibody functionalized AuNP-PDOP@carbon nanotubes (CNT). The stable and robust film, PDOP, was used to immobilize biomolecules not only for the construction of the sensor platform, but also for the signal labeling. Sensitivity was greatly amplified by using the special platform of AuNP-PDOP and synthesizing horseradish peroxidase (HRP)-antibody (Ab(2)) functionalized AuNP-PDOP@carbon nanotubes (CNT). A linear response range of IL-6 from 4.0 to 8.0 × 10(2) pg mL(-1) with a low detection limit of 1.0 pg mL(-1) was obtained by the amperometry determination. Measurements of IL-6 in human serum gave excellent correlations with standard ELISA assays. Moreover, the immunosensor exhibited high selectivity, good reproducibility, and stability.
研究了一种基于金纳米粒子（AuNP）-聚多巴胺（PDOP）作为传感器平台和多酶-抗体功能化 AuNP-PDOP@碳纳米管（CNT）的双重放大机制的电化学免疫传感器，用于灵敏检测白细胞介素-6（IL-6）。稳定且坚固的薄膜 PDOP 不仅用于构建传感器平台，还用于信号标记，可用于固定生物分子。通过使用 AuNP-PDOP 的特殊平台和合成辣根过氧化物酶（HRP）-抗体（Ab(2)）功能化 AuNP-PDOP@碳纳米管（CNT），大大提高了灵敏度。通过电流测定法，获得了 IL-6 的线性响应范围为 4.0 至 8.0×10(2) pg mL(-1)，检测限低至 1.0 pg mL(-1)。用该免疫传感器测定人血清中的 IL-6，与标准 ELISA 测定法具有极好的相关性。此外，该免疫传感器还表现出高选择性、良好的重现性和稳定性。
