Institute of Biomaterials, College of Sciences, South China Agricultural University, Guangzhou 510642, China.
Biosens Bioelectron. 2013 Jul 15;45:82-8. doi: 10.1016/j.bios.2013.01.058. Epub 2013 Feb 9.
An electrochemical, signal amplified immunosensor was developed to detect 3-bromobiphenyl (BBP) by using a bio-inspired polydopamine (PDOP)/gold nanocluster (AuNc) as the sensor platform and multienzyme-labeled carbon hollow nanochains as the signal amplifier. The self-polymerized dopamine membrane on the AuNc-modified indium tin oxide (ITO) electrode were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), contact angle and electrochemical measurements. Such PDOP/AuNc platform featured the mild cross-linking reaction with the dense immobilization of BBP-antigens (BBP-Ag). Moreover, by using multiple horseradish peroxidase (HRP) and secondary antibodies (Ab2) modified one-dimensional carbon hollow nanochains (CHNc) as the signal enhancer, it held promise for improving the sensitivity and detection limit of the immunoassay. Based on the competitive immunoassay protocol, this immunosensor showed a linear range from 1 pM to 2 nM for BBP with a detection limit of 0.5 pM. Also, it exhibited high sensitivity, wide linear range, acceptable stability and reproducibility on a promising immobilization platform using a novel signal amplifier, which may extend its application in other environmental monitoring.
电化学信号放大免疫传感器的设计原理是利用仿生聚多巴胺（PDOP）/金纳米簇（AuNc）作为传感器平台，以及多酶标记的碳空心纳米链作为信号放大器来检测 3-溴联苯（BBP）。在 AuNc 修饰的氧化铟锡（ITO）电极上自聚合的多巴胺膜通过扫描电子显微镜（SEM）、原子力显微镜（AFM）、接触角和电化学测量进行了表征。该 PDOP/AuNc 平台具有温和的交联反应，可实现 BBP 抗原（BBP-Ag）的密集固定。此外，通过使用多个辣根过氧化物酶（HRP）和二级抗体（Ab2）修饰的一维碳空心纳米链（CHNc）作为信号增强剂，有望提高免疫分析的灵敏度和检测限。基于竞争免疫分析方案，该免疫传感器对 BBP 的线性范围为 1 pM 至 2 nM，检测限为 0.5 pM。此外，它在使用新型信号放大器的有前途的固定化平台上表现出高灵敏度、宽线性范围、良好的稳定性和重现性，这可能会扩展其在其他环境监测中的应用。
