Hicke L, Schekman R
Division of Biochemistry and Molecular Biology, University of California, Berkeley 94720.
Bioessays. 1990 Jun;12(6):253-8. doi: 10.1002/bies.950120602.
The cellular machinery responsible for conveying proteins between the endoplasmic reticulum and the Golgi is being investigated using genetics and biochemistry. A role for vesicles in mediating protein traffic between the ER and the Golgi has been established by characterizing yeast mutants defective in this process, and by using recently developed cell-free assays that measure ER to Golgi transport. These tools have also allowed the identification of several proteins crucial to intracellular protein trafficking. The characterization and possible functions of several GTP-binding proteins, peripheral membrane proteins, and an integral membrane protein during ER to Golgi transport are discussed here.
目前正在通过遗传学和生物化学方法研究负责在内质网和高尔基体之间转运蛋白质的细胞机制。通过对在此过程中存在缺陷的酵母突变体进行表征，以及使用最近开发的用于测量从内质网到高尔基体转运的无细胞检测方法，已确定了囊泡在内质网和高尔基体之间介导蛋白质运输中的作用。这些工具还使得鉴定出几种对细胞内蛋白质运输至关重要的蛋白质成为可能。本文将讨论几种GTP结合蛋白、外周膜蛋白和一种整合膜蛋白在内质网到高尔基体运输过程中的特性及可能的功能。
