Johnson S A, Milner M J
Department of Biology and Preclinical Medicine, University of St. Andrews, Scotland, United Kingdom.
Int J Dev Biol. 1990 Jun;34(2):299-307.
We have cultured Drosophila wing imaginal discs in vitro under a variety of hormonal conditions in order to determine whether cuticle secretion is enhanced by a withdrawal of 20-hydroxy ecdysone at one of two points in development, corresponding to the drop in hormone titer during the prepupal period, and to the fall in hormone levels during the later stages if imaginal differentiation. We found that these treatments did not enhance either pupal or adult cuticle secretion.
我们在多种激素条件下对果蝇翅成虫盘进行了体外培养，以确定在发育的两个时间点之一去除20-羟基蜕皮激素是否会增强表皮分泌，这两个时间点分别对应于蛹前期激素滴度的下降，以及成虫分化后期激素水平的下降。我们发现，这些处理并未增强蛹期或成虫期的表皮分泌。
