Liang Feifei, Yang Yu, Zhao Yimin, Yu Nengjiang, Wang Jinhui
Institute of Pharmacology and Toxicology, Academy of Military Medical Science, Beijing 100850, China.
Zhongguo Zhong Yao Za Zhi. 2010 Oct;35(19):2581-4.
A HPLC method was developed for simultaneouly quantitative analyses of aviprin (1), gosferol (2), 3'R-(+)-hamaudol (3), 3'-O-acetylhamaudol (4) and iso-imperatorin (5) in the rhizome of Angelica polymorpha. The analysis was performed at 25 degrees C on an Agilent Eclipse XDB-C18 analytical column (4.6 mm x 250 mm, 5 microm) with the mobile phase of methanol and H2O in gradient elution [55:45 (0 min)-65:35 (25 min)-95:5 (35 min)]. And the flow rate was 1.0 mL min(-1) with the detection wavelengths of 312 nm (1), 306 nm (2), 300 nm (3), 294 nm (4) and 310 nm (5). Consequently, the reqresion equations were Y = 1.81 x 10(3) X + 7.93 x 10(2) (r = 0.9996), Y = 2.49 x 10(3) X - 2.17 x 10(2) (r = 0.9993), Y = 2.02 x 10(3) X - 1.42 x 10(2) (r = 0.9991), Y = 1.57 x 10(3) X - 0.66 x 10(2) (r = 0.999 7), Y = 2.65 x 10(3) X - 1.47 x 10(2) (r = 0.999 6). And the average recoveries were 99.7% (RSD 0.57%), 100.1% (RSD 1.3%), 100.0% (RSD 1.6%), 99.6% (RSD 1.3%), 99.2% (RSD 0.59%), respectively. The precision, repeatability and stability were all consistent with the request of quantitative analysis. The contents of compound 1-5 in A. polymorpha were determined as 0.525%, 0.044%, 0.046%, 0.043%, 0.15%, respectively. Accordingly, this quantitative analysis method is good for the quality control of A. polymorpha.
建立了一种高效液相色谱法，用于同时定量分析多形当归根茎中的阿维菌素（1）、孕甾醇（2）、3'R-(+)-哈马豆醇（3）、3'-O-乙酰哈马豆醇（4）和异欧前胡素（5）。分析在25℃下于Agilent Eclipse XDB-C18分析柱（4.6 mm×250 mm，5μm）上进行，流动相为甲醇和水，采用梯度洗脱[55:45（0分钟）-65:35（25分钟）-95:5（35分钟）]。流速为1.0 mL min⁻¹，检测波长分别为312 nm（1）、306 nm（2）、300 nm（3）、294 nm（4）和310 nm（5）。结果得到回归方程分别为Y = 1.81×10³X + 7.93×10²（r = 0.9996）、Y = 2.49×10³X - 2.17×10²（r = 0.9993）、Y = 2.02×10³X - 1.42×10²（r = 0.9991）、Y = 1.57×10³X - 0.66×10²（r = 0.9997）、Y = 2.65×10³X - 1.47×10²（r = 0.9996）。平均回收率分别为99.7%（相对标准偏差0.57%）、100.1%（相对标准偏差1.3%）、100.0%（相对标准偏差1.6%）、99.6%（相对标准偏差1.3%）、99.2%（相对标准偏差0.59%）。精密度、重复性和稳定性均符合定量分析要求。多形当归中化合物1 - 5的含量分别测定为0.525%、0.044%、0.046%、0.043%、0.15%。因此，该定量分析方法有利于多形当归的质量控制。
