Anal Chem. 2011 Feb 1;83(3):648-53. doi: 10.1021/ac103047c. Epub 2010 Dec 22.
Functionalized graphene oxide (GO) sheets coupled with a signal amplification method based on the nanomaterial-promoted reduction of silver ions for the sensitive and selective detection of bacteria. This paper aims to develop an electrochemical route combined with GO sheet-mediated Ag enhancement for biological/chemical analyte detection. A linear relationship between the stripping response and the logarithm of the bacterial concentration was obtained using an electrochemical technique for concentrations ranging from 1.8 × 10(2) to 1.8 × 10(8) cfu mL(-1), with a slope of 15.28 and a correlation coefficient of 0.995. Dot blot assay was used as a conventional immunoassay method for comparison with the electrochemical method, as well as to observe the quality of the anti-sulfate-reducing bacteria (SRB) antibody (Ab) used in the immunosensor. The GO sheet-mediated silver enhancement holds great potential for the rapid analysis of protein, DNA, and pathogens.
功能化氧化石墨烯 (GO) 片与基于纳米材料促进银离子还原的信号放大方法相结合，用于细菌的灵敏和选择性检测。本文旨在开发一种电化学方法，结合 GO 片介导的 Ag 增强，用于生物/化学分析物的检测。通过电化学技术获得了从 1.8×10(2)到 1.8×10(8) cfu mL(-1)范围内的细菌浓度与剥离响应之间的线性关系，斜率为 15.28，相关系数为 0.995。点印迹分析被用作与电化学方法进行比较的传统免疫分析方法，以及观察用于免疫传感器的抗硫酸盐还原菌 (SRB) 抗体 (Ab) 的质量。GO 片介导的银增强在快速分析蛋白质、DNA 和病原体方面具有很大的潜力。
