Song Y, Zhang L S, Wang H, Jin H, Li Ch, Jin N
Tianjin University, Tianjin, People's Republic of China.
Acta Virol. 2010;54(4):293-6. doi: 10.4149/av_2010_04_293.
Human immunodeficiency viruses 1 and 2 (HIV-1, 2) present a public health problem for which there is neither an effective antiviral therapy nor a preventive vaccine. In this study, the immune responses of mice to prime-boost vaccination with the recombinant DNA (rDNA) and recombinant Fowlpox virus (rFPV) both expressing HIV-2 Gag-gp105 chimeric protein, were compared to those elicited by each vector alone. Mice primed with the rDNA and boosted with the rFPV showed HIV-2-specific antibody levels, splenic CD4+ and CD8+ T-lymphocyte numbers, and Gag-gp105-specific cytotoxic T-lymphocytes (CTL) activity increased by 20-30% as compared with those elicited by these vaccines alone. These findings suggested that the prime-boost strategy combining rDNA and rFPV elicited significant Gag-gp105 - specific cellular and humoral immune responses, thus supporting this novel approach to the immunization against HIV infections.
人类免疫缺陷病毒1型和2型（HIV - 1、2）构成了一个公共卫生问题，针对这一问题，既没有有效的抗病毒疗法，也没有预防性疫苗。在本研究中，将小鼠对同时用表达HIV - 2 Gag - gp105嵌合蛋白的重组DNA（rDNA）和重组鸡痘病毒（rFPV）进行初免 - 加强免疫接种的免疫反应，与单独使用每种载体引发的免疫反应进行了比较。用rDNA初免并用rFPV加强免疫的小鼠，其HIV - 2特异性抗体水平、脾脏CD4⁺和CD8⁺ T淋巴细胞数量以及Gag - gp105特异性细胞毒性T淋巴细胞（CTL）活性，与单独使用这些疫苗引发的相比，增加了20% - 30%。这些发现表明，结合rDNA和rFPV的初免 - 加强策略引发了显著的Gag - gp105特异性细胞免疫和体液免疫反应，从而支持了这种针对HIV感染免疫的新方法。
