Inserm, U-1003, Equipe labellisée par Ligue Nationale contre cancer, Université de Lille-1 (USTL), Villeneuve d'Ascq, France.
J Eur Acad Dermatol Venereol. 2011 Feb;25 Suppl 1:12-8. doi: 10.1111/j.1468-3083.2010.03894.x.
Numerous studies have demonstrated the beneficial effect of Avène Thermal Spring Water (TSW) in dermatological diseases but the molecular mechanisms remain unknown. The objective of the present study was to evaluate the effect of Avène TSW on the morphological and molecular features related to the more advanced status of differentiation of human keratinocytes.
Normal human keratinocytes (NHK) were differentiated in medium powder reconstituted with Avène TSW and assessed by RT-PCR and immunohistochemistry. Calcium entry was measured by a Fura-2 AM probe. TRPV6 channel were detected by immunohistochemistry, RT-PCR and western blot.
Treatment of NHK with Avène TSW led to an enhanced constitutive calcium entry that resulted in the increased expression of involucrin and cytokeratins 1 and 10. This enhanced constitutive calcium entry in Avène TSW-treated keratinocytes was mediated by the TRPV6 calcium channel. Moreover, Avène TSW-mediated calcium entry was due to the increase in TRPV6 expression as well as the channel abundance at the cell membrane.
An other mechanism of action of Avène TSW is described. Avène TSW treatment induced an enhanced constitutive calcium entry mediated by TRPV6 channel leading to the acceleration of human keratinocytes differentiation.
许多研究表明雅漾温泉水（TSW）对皮肤科疾病有益，但分子机制尚不清楚。本研究的目的是评估雅漾 TSW 对与人类角质形成细胞分化更高级状态相关的形态和分子特征的影响。
用雅漾 TSW 重建的培养基粉末使正常人角质形成细胞（NHK）分化，并通过 RT-PCR 和免疫组织化学进行评估。通过 Fura-2 AM 探针测量钙内流。通过免疫组织化学、RT-PCR 和 Western blot 检测 TRPV6 通道。
用雅漾 TSW 处理 NHK 可导致组成性钙内流增强，从而导致内披蛋白和角蛋白 1 和 10 的表达增加。雅漾 TSW 处理的角质形成细胞中的这种增强的组成性钙内流是由 TRPV6 钙通道介导的。此外，雅漾 TSW 介导的钙内流是由于 TRPV6 表达的增加以及细胞膜上通道丰度的增加。
描述了雅漾 TSW 的另一种作用机制。雅漾 TSW 处理诱导了由 TRPV6 通道介导的增强的组成性钙内流，从而加速了人类角质形成细胞的分化。
