Klimanova G V, Petrosian E A, Rostovtseva N A
Zh Mikrobiol Epidemiol Immunobiol. 1978 Jul(7):108-12.
Data presented in this work indicated that antigens, contrastive by toxicity, obtained by Boiven's method and O'Neill and Tood's method from two strains of Bordetella pertussis differed by stability of lipid A binding with the specific polysaccharide. The influence of duration of the lipopolysaccharide hydrolysis on the fatty acid content in lipid A, and of heptose in the specific polysaccharide was demonstrated. Lipid A fatty acid composition was studied. It is supposed that bound fatty acids are presented as C14 and C19--C22. There was a correlation between the antigen toxicity and the stability of lipid A bond with the specific polysaccharide. Stability of the lipopolysaccharide complex bond depended on heptose and lipid A content and on the composition and the amount of fatty acids in the lipid A preparations.
本研究中呈现的数据表明，通过博伊文氏法以及奥尼尔和托德氏法从两株百日咳博德特氏菌中获得的、按毒性对比的抗原，在脂多糖A与特异性多糖结合的稳定性方面存在差异。研究证明了脂多糖水解时间对脂多糖A中脂肪酸含量以及特异性多糖中庚糖含量的影响。对脂多糖A的脂肪酸组成进行了研究。据推测，结合脂肪酸以C14以及C19 - C22的形式存在。抗原毒性与脂多糖A与特异性多糖的结合稳定性之间存在相关性。脂多糖复合物键的稳定性取决于庚糖和脂多糖A的含量，以及脂多糖A制剂中脂肪酸的组成和含量。
