Lin Xian-dong, Li Chao, Shi Yi, Chen Yan, Zhang Li-yuan, Zheng Xiong-wei
Department of Pathology, Fujian Provincial Tumor Hospital, the Teaching Hospital of Fujian Medical University, Fuzhou 350014, China.
Zhonghua Bing Li Xue Za Zhi. 2010 Oct;39(10):681-5.
To explore the correlation of functional genetic variants in Nme1-509 C>T and TGFβ1-1465 T>C genes to the genetic susceptibility of gastric carcinoma in Fujian province, China.
A case-control study was conducted in a population in Fujian province. The polymorphism of TGFβ1-509 C>T (rs1800469), Nme1-1465 T>C (rs16949649) in 273 gastric carcinoma patients and 277 cancer-free controls, frequency-matched by age and sex, were analysed by using Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF-MS). Adjusted odds ratios (OR) and 95% confidence evaluation intervals (95%CI) measured by multivariate Logistic regression analysis were adopted in studying the correlation of the gene polymorphism with the susceptibility of gastric cancer.
After the adjustment using Logistic regression or the potential confounding effects of gender and age, as compared with TT+CT genotype gastric carcinoma patients, the homozygous Nme1-1465CC genotype carriers had a significantly higher risk in lymph node metastasis, with the OR of 2.5 (95%CI 0.08-2.10; P=0.029). There was no association obtained between TGFβ1-509 T>C genotype with the tumor size, cell differentiation, tumor invasion and lymph node metastasis in gastric carcinoma. In the intestinal type gastric carcinoma group, when compared with the wild homozygous Nme1 TT* TGFβ1 CC, Nme1 TC* TGFβ1 TC, Nme1 TC* TGFβ1 TT and Nme1 CC* TGFβ1 TC genotype carriers, there was a significantly decrease of risk in gastric carcinogenesis of 0.42 fold (95%CI 0.54-0.94, P=0.022), 0.32 fold (95%CI 0.42-0.97, P=0.013) and 0.26 fold (95%CI 0.42-0.97, P=0.008), respectively.
There is a significant relationship between polymorphism of Nme1-1465 T>C and the prognosis of carcinoma of stomach. It also demonstrates that coexistence of Nme1-1465 T>C and TGFβ1-509 T>C genes may provide a synergistic effect of increasing the susceptibility of gastric carcinogenesis.
探讨Nme1基因-509C>T和TGFβ1基因-1465T>C功能基因变异与中国福建省胃癌遗传易感性的相关性。
在福建省人群中进行病例对照研究。采用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）分析273例胃癌患者和277例年龄、性别频率匹配的无癌对照者中TGFβ1基因-509C>T（rs1800469）、Nme1基因-1465T>C（rs16949649）的多态性。采用多因素Logistic回归分析计算校正比值比（OR）和95%可信区间（95%CI），研究基因多态性与胃癌易感性的相关性。
经Logistic回归调整或校正性别和年龄的潜在混杂效应后，与TT+CT基因型胃癌患者相比，Nme1基因-1465CC纯合基因型携带者发生淋巴结转移的风险显著更高，OR为2.5（95%CI 0.08-2.10；P=0.029）。未发现TGFβ1基因-509T>C基因型与胃癌的肿瘤大小、细胞分化、肿瘤浸润及淋巴结转移之间存在关联。在肠型胃癌组中，与野生纯合Nme1 TTTGFβ1 CC、Nme1 TCTGFβ1 TC、Nme1 TCTGFβ1 TT和Nme1 CCTGFβ1 TC基因型携带者相比，胃癌发生风险分别显著降低0.42倍（95%CI 0.54-0.94，P=0.022）、0.32倍（95%CI 0.42-0.97，P=0.013）和0.26倍（95%CI 0.42-0.97，P=0.008）。
Nme1基因-1465T>C多态性与胃癌预后存在显著相关性。同时表明Nme1基因-1465T>C和TGFβ1基因-509T>C共存可能具有协同增加胃癌发生易感性的作用。
